cDNA cloning and genomic organization of peroxisome proliferator-inducible long-chain acyl-CoA hydrolase from rat liver cytosol. 1998

J Yamada, and K Suga, and T Furihata, and M Kitahara, and T Watanabe, and M Hosokawa, and T Satoh, and T Suga
Department of Clinical Biochemistry, Tokyo University of Pharmacy and Life Science, Japan. junymd@ps.toyaku.ac.jp

The cDNA for a peroxisome proliferator-inducible long-chain acyl-CoA hydrolase from rat liver cytosol, referred to as rLACH2, was isolated and its genomic structure was determined. The cDNA encoded a 419-amino-acid polypeptide with a calculated molecular weight of 46,011. Sequence analysis identified an active-site serine motif (Gly-x-Ser-x-Gly) common to carboxylesterases and lipases. When expressed in Escherichia coli, the cDNA directed expression of a protein immunoreactive to an anti-rLACH2 antibody with a molecular mass of 47 kDa, identical to that of purified rLACH2. Northern blot analysis showed marked induction of rLACH2 mRNA in the liver after feeding rats with di(2-ethylhexyl)phthalate, a peroxisome proliferator. The rLACH2 gene spanned about 19 kb and comprised 3 exons, the intron/exon boundaries of which were consistent with the donor/acceptor splice rule. A putative peroxisome proliferator response element (AGGTCATGGTTCA) was identified in the 5'-flanking region, suggesting the involvement of peroxisome proliferator-activated receptors in the regulation of rLACH2 gene expression.

UI MeSH Term Description Entries
D007438 Introns Sequences of DNA in the genes that are located between the EXONS. They are transcribed along with the exons but are removed from the primary gene transcript by RNA SPLICING to leave mature RNA. Some introns code for separate genes. Intervening Sequences,Sequences, Intervening,Intervening Sequence,Intron,Sequence, Intervening
D008099 Liver A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances. Livers
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D008970 Molecular Weight The sum of the weight of all the atoms in a molecule. Molecular Weights,Weight, Molecular,Weights, Molecular
D010170 Palmitoyl-CoA Hydrolase Enzyme catalyzing reversibly the hydrolysis of palmitoyl-CoA or other long-chain acyl coenzyme A compounds to yield CoA and palmitate or other acyl esters. The enzyme is involved in the esterification of fatty acids to form triglycerides. EC 3.1.2.2. Acyl CoA Hydrolase,Fatty Acyl Thioesterase,Palmitoyl CoA Deacylase,Palmitoyl Coenzyme A Hydrolase,Palmitoyl Thioesterase,Long-Chain Fatty-Acyl-CoA Hydrolase,Oleoyl-CoA Acylhydrolase,Stearoyl CoA Hydrolase,Thioesterase I,Acylhydrolase, Oleoyl-CoA,CoA Deacylase, Palmitoyl,CoA Hydrolase, Acyl,CoA Hydrolase, Stearoyl,Deacylase, Palmitoyl CoA,Fatty-Acyl-CoA Hydrolase, Long-Chain,Hydrolase, Acyl CoA,Hydrolase, Long-Chain Fatty-Acyl-CoA,Hydrolase, Palmitoyl-CoA,Hydrolase, Stearoyl CoA,Long Chain Fatty Acyl CoA Hydrolase,Oleoyl CoA Acylhydrolase,Palmitoyl CoA Hydrolase,Thioesterase, Fatty Acyl,Thioesterase, Palmitoyl
D011994 Recombinant Proteins Proteins prepared by recombinant DNA technology. Biosynthetic Protein,Biosynthetic Proteins,DNA Recombinant Proteins,Recombinant Protein,Proteins, Biosynthetic,Proteins, Recombinant DNA,DNA Proteins, Recombinant,Protein, Biosynthetic,Protein, Recombinant,Proteins, DNA Recombinant,Proteins, Recombinant,Recombinant DNA Proteins,Recombinant Proteins, DNA
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D003600 Cytosol Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components. Cytosols
D004051 Diethylhexyl Phthalate An ester of phthalic acid. It appears as a light-colored, odorless liquid and is used as a plasticizer for many resins and elastomers. Dioctyl Phthalate,Bis(2-ethylhexyl)phthalate,DEHP,Di(2-ethylhexyl)phthalate,Di-2-Ethylhexylphthalate,Di 2 Ethylhexylphthalate,Phthalate, Diethylhexyl,Phthalate, Dioctyl
D004790 Enzyme Induction An increase in the rate of synthesis of an enzyme due to the presence of an inducer which acts to derepress the gene responsible for enzyme synthesis. Induction, Enzyme

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