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Conclusive evidence has been obtained for the presence of both hepatic and extrahepatic triglyceride lipase activities (TGLA) in rat postheparin plasma, and an assay has been devised for their selective measurement. Heparin-released TGLA in plasma from the intact rat, like TGLA in postheparin hepatic perfusate, was relatively resistant to inactivation by salt and protamine. Postheparin TGLA obtained from the supradiaphragmatic portion of the rat, where any hepatic contribution was eliminated, was nearly completely inactivated by salt and protamine. Utilizing the different sensitivities to protamine inactivation of extrahepatic and hepatic TGLA, assay conditions were selected to achieve simultaneously the maximal reduction of extrahepatic TGLA with preservation of hepatic TGLA. This assay was validated using postheparin plasma from partially hepatectomized rats. The protamine-inactivated activity was independent of the amount of liver removed, whereas protamine-resistant activity was directly proportional to the amount of liver remaining. In the intact rat, liver appeared to be the major source of heparin-released TGLA measured at pH 8.6 with triolein substrate. It was further shown that both hepatic and extrahepatic lipases catalyzed hydrolysis of triglyceride-rich lipoproteins.
R M Krauss, and
H G Windmueller, and
R I Levy, and
D S Fredrickson
July 1987,
Chemical & pharmaceutical bulletin,
R M Krauss, and
H G Windmueller, and
R I Levy, and
D S Fredrickson
January 1984,
Advances in experimental medicine and biology,
R M Krauss, and
H G Windmueller, and
R I Levy, and
D S Fredrickson
January 1981,
Methods in enzymology,
R M Krauss, and
H G Windmueller, and
R I Levy, and
D S Fredrickson
November 1974,
The Journal of clinical investigation,
R M Krauss, and
H G Windmueller, and
R I Levy, and
D S Fredrickson
February 1984,
Metabolism: clinical and experimental,
R M Krauss, and
H G Windmueller, and
R I Levy, and
D S Fredrickson
August 1980,
Metabolism: clinical and experimental,
R M Krauss, and
H G Windmueller, and
R I Levy, and
D S Fredrickson
January 1991,
Methods in enzymology,
R M Krauss, and
H G Windmueller, and
R I Levy, and
D S Fredrickson
December 1983,
Clinical chemistry,
R M Krauss, and
H G Windmueller, and
R I Levy, and
D S Fredrickson
February 1977,
Metabolism: clinical and experimental,
R M Krauss, and
H G Windmueller, and
R I Levy, and
D S Fredrickson
September 1975,
Clinica chimica acta; international journal of clinical chemistry,
