-transparent.png){kind=logo}
The presence of hCG beta-core fragment (beta-core) in the human placenta has been controversial. To clarify its presence in the villous tissue, first, we developed an enzyme immunoassay which is highly specific for beta-core. Then, we investigated the presence of beta-core immunoreactivity in the supernatants of placental organ culture and those of primary culture of trophoblasts as well as in the placental extracts at different stages of gestation. The immunoreactivity of beta-core was demonstrated in each sample, and the amount was at least 5% of intact hCG immunoreactivity. Immunohistochemical analysis of the placental tissue showed localization of beta-core immunoreactivity to the syncytiotrophoblast. Western blot analysis of the supernatants of organ culture as well as urine samples of pregnant women showed two major bands with molecular weight of approximately 15000. On the other hand, beta-core immunoreactivity in the pregnant sera ranged from 0.1 to 0.19% of intact hCG immunoreactivity. These results suggest the presence and possible secretion of beta-core fragment in the human placenta, and its immunoreactivity in the serum is likely to be reduced due to its short half life in the maternal blood.
A Udagawa, and
T Okamoto, and
S Nomura, and
K Matsuo, and
H Suzuki, and
S Mizutani
July 2010,
Nihon rinsho. Japanese journal of clinical medicine,
A Udagawa, and
T Okamoto, and
S Nomura, and
K Matsuo, and
H Suzuki, and
S Mizutani
August 2005,
Nihon rinsho. Japanese journal of clinical medicine,
A Udagawa, and
T Okamoto, and
S Nomura, and
K Matsuo, and
H Suzuki, and
S Mizutani
December 1999,
Nihon rinsho. Japanese journal of clinical medicine,
A Udagawa, and
T Okamoto, and
S Nomura, and
K Matsuo, and
H Suzuki, and
S Mizutani
January 1995,
Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association,
A Udagawa, and
T Okamoto, and
S Nomura, and
K Matsuo, and
H Suzuki, and
S Mizutani
January 2001,
Life sciences,
A Udagawa, and
T Okamoto, and
S Nomura, and
K Matsuo, and
H Suzuki, and
S Mizutani
September 1988,
Molecular endocrinology (Baltimore, Md.),
A Udagawa, and
T Okamoto, and
S Nomura, and
K Matsuo, and
H Suzuki, and
S Mizutani
April 1993,
Gynecologic oncology,
A Udagawa, and
T Okamoto, and
S Nomura, and
K Matsuo, and
H Suzuki, and
S Mizutani
June 1988,
The Journal of clinical endocrinology and metabolism,
A Udagawa, and
T Okamoto, and
S Nomura, and
K Matsuo, and
H Suzuki, and
S Mizutani
January 2000,
Placenta,
A Udagawa, and
T Okamoto, and
S Nomura, and
K Matsuo, and
H Suzuki, and
S Mizutani
March 1988,
The Journal of clinical endocrinology and metabolism,
