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Correct folding of newly synthesized polypeptides is thought to be facilitated by Hsp70 molecular chaperones in conjunction with DnaJ cohort proteins. In Saccharomyces cerevisiae, SSB proteins are ribosome-associated Hsp70s which interact with the newly synthesized nascent polypeptide chain. Here we report that the phenotypes of an S.cerevisiae strain lacking the DnaJ-related protein Zuotin (Zuo1) are very similar to those of a strain lacking Ssb, including sensitivities to low temperatures, certain protein synthesis inhibitors and high osmolarity. Zuo1, which has been shown previously to be a nucleic acid-binding protein, is also a ribosome-associated protein localized predominantly in the cytosol. Analysis of zuo1 deletion and truncation mutants revealed a positive correlation between the ribosome association of Zuo1 and its ability to bind RNA. We propose that Zuo1 binds to ribosomes, in part, by interaction with ribosomal RNA and that Zuo1 functions with Ssb as a chaperone on the ribosome.
W Yan, and
B Schilke, and
C Pfund, and
W Walter, and
S Kim, and
E A Craig
November 2006,
Biochemical and biophysical research communications,
W Yan, and
B Schilke, and
C Pfund, and
W Walter, and
S Kim, and
E A Craig
March 2001,
Proceedings of the National Academy of Sciences of the United States of America,
W Yan, and
B Schilke, and
C Pfund, and
W Walter, and
S Kim, and
E A Craig
April 2024,
Nucleic acids research,
W Yan, and
B Schilke, and
C Pfund, and
W Walter, and
S Kim, and
E A Craig
August 2011,
Fish & shellfish immunology,
W Yan, and
B Schilke, and
C Pfund, and
W Walter, and
S Kim, and
E A Craig
December 1994,
Proceedings of the National Academy of Sciences of the United States of America,
W Yan, and
B Schilke, and
C Pfund, and
W Walter, and
S Kim, and
E A Craig
January 2021,
Magnetic resonance (Gottingen, Germany),
W Yan, and
B Schilke, and
C Pfund, and
W Walter, and
S Kim, and
E A Craig
February 1994,
Proceedings of the National Academy of Sciences of the United States of America,
W Yan, and
B Schilke, and
C Pfund, and
W Walter, and
S Kim, and
E A Craig
March 2001,
FEMS microbiology letters,
W Yan, and
B Schilke, and
C Pfund, and
W Walter, and
S Kim, and
E A Craig
December 2006,
Biological chemistry,
W Yan, and
B Schilke, and
C Pfund, and
W Walter, and
S Kim, and
E A Craig
June 1997,
Journal of molecular biology,
