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The ability of monoclonal based dot-ELISA and a previously described solid-phase ELISA were directly compared to detect circulating schistosomal antigens in sera from 50 Egyptian individuals with parasitologically proven schistosomiasis. The mAb employed, 128C3/3/21, recognized a repeating carbohydrate epitope expressed at all stages of the parasite development. The same mAb and patient serum samples were used i both tests, 17 samples from age-matched individuals infected with other parasites but not with Schistosoma were also tested. In the dot-ELISA, 44 serum samples (88%) were found to contain parasite antigens when screen at a dilution of 1;24, 43 serum samples (86%) were positive in the solid-phase ELISA at 1:8 dilution and only 37 (74%) samples were positive at 1:24 dilution. The specificity of the dot-ELISA was 93% as compared to > 99% for the solid-phase ELISA. The detection limit of dot-ELISA was 0.01 ng/ml, whereas only > 1 ng/ml could be detected by the solid-phase ELISA. As dot-ELISA does not require radioactivity or sophisticated equipment, so it is practically well suited for use in endemic areas in developing countries.
M M Hassan, and
N E Mostafa, and
N Abbas, and
M Shalaby, and
S Mansour
January 1995,
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases,
M M Hassan, and
N E Mostafa, and
N Abbas, and
M Shalaby, and
S Mansour
September 1986,
Lancet (London, England),
M M Hassan, and
N E Mostafa, and
N Abbas, and
M Shalaby, and
S Mansour
January 1988,
Transactions of the Royal Society of Tropical Medicine and Hygiene,
M M Hassan, and
N E Mostafa, and
N Abbas, and
M Shalaby, and
S Mansour
April 1985,
Infection and immunity,
M M Hassan, and
N E Mostafa, and
N Abbas, and
M Shalaby, and
S Mansour
January 1989,
The American journal of tropical medicine and hygiene,
M M Hassan, and
N E Mostafa, and
N Abbas, and
M Shalaby, and
S Mansour
February 1975,
British medical journal,
M M Hassan, and
N E Mostafa, and
N Abbas, and
M Shalaby, and
S Mansour
August 1975,
British medical journal,
M M Hassan, and
N E Mostafa, and
N Abbas, and
M Shalaby, and
S Mansour
June 1988,
Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao,
M M Hassan, and
N E Mostafa, and
N Abbas, and
M Shalaby, and
S Mansour
December 1987,
Kisaengch'unghak chapchi. The Korean journal of parasitology,
M M Hassan, and
N E Mostafa, and
N Abbas, and
M Shalaby, and
S Mansour
January 1990,
Zhonghua yi xue za zhi,
