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The isolation of large DNA fragments (> 5 kb) from agarose gels can be problematic. The DNA yield is often low, and the purification may be insufficient for subsequent reactions such as sequencing or ligation. Here we have compared a number of commonly used methods and commercial kits for DNA recovery. Large DNA fragments (12 and 14 kb, respectively) were isolated from agarose gel and purified, the recovery yield was tested, and a well-defined amount of purified DNA was used in a standard ligation reaction. We observed dramatic differences in the efficiency of this ligation reaction depending on which recovery method had been used. However, the respective ligation efficiencies of the different methods were not related to their recovery yields.
E Fransen, and
G Van Camp, and
B Winnepenninckx
October 1994,
BioTechniques,
E Fransen, and
G Van Camp, and
B Winnepenninckx
May 2001,
Current protocols in immunology,
E Fransen, and
G Van Camp, and
B Winnepenninckx
August 2002,
Current protocols in molecular biology,
E Fransen, and
G Van Camp, and
B Winnepenninckx
December 1995,
Nucleic acids research,
E Fransen, and
G Van Camp, and
B Winnepenninckx
April 1999,
Analytical biochemistry,
E Fransen, and
G Van Camp, and
B Winnepenninckx
January 1996,
Methods in molecular biology (Clifton, N.J.),
E Fransen, and
G Van Camp, and
B Winnepenninckx
April 1995,
Molecular biotechnology,
E Fransen, and
G Van Camp, and
B Winnepenninckx
June 2001,
Analytical biochemistry,
E Fransen, and
G Van Camp, and
B Winnepenninckx
April 1988,
BioTechniques,
E Fransen, and
G Van Camp, and
B Winnepenninckx
April 1981,
Analytical biochemistry,
