BIOMAT Database Logo BIOMAT Database Logo

Detection of Lawsonia intracellularis in Hungarian swine herds by polymerase chain reaction. 1998

I Biksi, and I Kacskovics, and M Mándoki, and J Iván, and I Horváth-Papp, and G Makay, and F Vetési
Department of Pathology and Forensic Veterinary Medicine, University of Veterinary Science, Budapest, Hungary.

Pooled faecal samples and/or intestinal contents from pig carcasses were collected from 11 Hungarian swine farms and subjected to DNA extraction/ purification and subsequent polymerase chain reaction (PCR) in order to detect Lawsonia intracellularis, the aetiological agent of the porcine proliferative enteropathy complex. Specific PCR positivity was detected in 6 individual and 22 pooled samples out of 46, originating from eight herds. The PCR products of collected faecal samples hybridised in Southern blot hybridisation with the DNA of the type strain L. intracellularis NCTC 12657. This is the first confirmed detection of L. intracellularis by PCR in Hungary.

UI MeSH Term Description Entries
D011247 Pregnancy The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH. Gestation,Pregnancies
D005260 Female Females
D006090 Gram-Negative Bacteria Bacteria which lose crystal violet stain but are stained pink when treated by Gram's method. Gram Negative Bacteria
D006814 Hungary A country in Europe, northwest of Romania, south of Slovakia, and east of Austria. The capital is Budapest.
D006824 Hybridization, Genetic The genetic process of crossbreeding between genetically dissimilar parents to produce a hybrid. Crossbreeding,Hybridization, Intraspecies,Crossbreedings,Genetic Hybridization,Genetic Hybridizations,Hybridizations, Genetic,Hybridizations, Intraspecies,Intraspecies Hybridization,Intraspecies Hybridizations
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013552 Swine Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA). Phacochoerus,Pigs,Suidae,Warthogs,Wart Hogs,Hog, Wart,Hogs, Wart,Wart Hog
D013553 Swine Diseases Diseases of domestic swine and of the wild boar of the genus Sus. Disease, Swine,Diseases, Swine,Swine Disease
D015139 Blotting, Southern A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES. Southern Blotting,Blot, Southern,Southern Blot
D016133 Polymerase Chain Reaction In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain

Related Publications

I Biksi, and I Kacskovics, and M Mándoki, and J Iván, and I Horváth-Papp, and G Makay, and F Vetési
Detection of Lawsonia intracellularis in swine using polymerase chain reaction methodology.
January 1999, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc,
I Biksi, and I Kacskovics, and M Mándoki, and J Iván, and I Horváth-Papp, and G Makay, and F Vetési
Simultaneous detection of Lawsonia intracellularis, Brachyspira hyodysenteriae and Salmonella spp. in swine intestinal specimens by multiplex polymerase chain reaction.
September 2005, Journal of veterinary science,
I Biksi, and I Kacskovics, and M Mándoki, and J Iván, and I Horváth-Papp, and G Makay, and F Vetési
Prevalence of Lawsonia intracellularis in swine herds in Taiwan.
July 1997, The Veterinary record,
I Biksi, and I Kacskovics, and M Mándoki, and J Iván, and I Horváth-Papp, and G Makay, and F Vetési
A quantitative polymerase chain reaction assay for detection and quantification of Lawsonia intracellularis.
March 2010, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc,
I Biksi, and I Kacskovics, and M Mándoki, and J Iván, and I Horváth-Papp, and G Makay, and F Vetési
Prevalence of Lawsonia intracellularis, Brachyspira hyodysenteriae and Salmonella in swine herds.
December 2005, Journal of veterinary science,
I Biksi, and I Kacskovics, and M Mándoki, and J Iván, and I Horváth-Papp, and G Makay, and F Vetési
Development of a polymerase chain reaction assay for quantification of Lawsonia intracellularis.
July 2010, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc,
I Biksi, and I Kacskovics, and M Mándoki, and J Iván, and I Horváth-Papp, and G Makay, and F Vetési
Evaluation of antemortem polymerase chain reaction and serologic methods for detection of Lawsonia intracellularis-exposed pigs.
June 1998, American journal of veterinary research,
I Biksi, and I Kacskovics, and M Mándoki, and J Iván, and I Horváth-Papp, and G Makay, and F Vetési
Diagnosis of porcine proliferative enteropathy: detection of Lawsonia intracellularis by pathological examinations, polymerase chain reaction and cell culture inoculation.
September 2003, Journal of veterinary medicine. B, Infectious diseases and veterinary public health,
I Biksi, and I Kacskovics, and M Mándoki, and J Iván, and I Horváth-Papp, and G Makay, and F Vetési
Multiplex polymerase chain reaction for simultaneous detection of Lawsonia intracellularis, Serpulina hyodysenteriae, and salmonellae in porcine intestinal specimens.
July 1997, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc,
I Biksi, and I Kacskovics, and M Mándoki, and J Iván, and I Horváth-Papp, and G Makay, and F Vetési
Immunohistochemistry and polymerase chain reaction for the detection of Lawsonia intracellularis in porcine intestinal tissues with proliferative enteropathy.
July 2000, The Journal of veterinary medical science,
Copied contents to your clipboard!