-transparent.png){kind=logo}
We screened 57 Sahiwal cattle (Bos indicus) and 53 Murrah, 19 Nili-Ravi and 11 Egyptian buffaloes (Bubalus bubalis) to detect the polymorphisms at kappa-casein (CSN3) gene using the polymerase chain reaction (PCR). CSN3 A and B alleles were identified by PCR-RFLP using the restriction enzymes that detect the underlying nucleotide changes at codon 136 (Taql) and at codon 148 (HindIII or HinfI) in cattle. The frequency of CSN3 B allele in the Sahiwal cattle was estimated as 0.16 with no homozygous BB animal. Using the same set of primers as used in the Sahiwal cattle, a part of exon IV of buffalo CSN3 gene was amplified, but restriction enzyme analysis using HindIII/HinfI and TaqI did not reveal any polymorphism. However, DNA sequencing of the amplified fragment (GenBank Acc. No. U96662) revealed one polymorphism at codon 135 (ThrACC -->I1eATC) in buffalo; the frequencies of 135 Thr/Ile alleles were estimated as 0.88 and 0.12, respectively.
A Mitra, and
P Schlee, and
I Krause, and
J Blusch, and
T Werner, and
C R Balakrishnan, and
F Pirchner
April 1996,
Animal genetics,
A Mitra, and
P Schlee, and
I Krause, and
J Blusch, and
T Werner, and
C R Balakrishnan, and
F Pirchner
December 2023,
Animal biotechnology,
A Mitra, and
P Schlee, and
I Krause, and
J Blusch, and
T Werner, and
C R Balakrishnan, and
F Pirchner
February 1972,
Biochemical genetics,
A Mitra, and
P Schlee, and
I Krause, and
J Blusch, and
T Werner, and
C R Balakrishnan, and
F Pirchner
April 1993,
Animal genetics,
A Mitra, and
P Schlee, and
I Krause, and
J Blusch, and
T Werner, and
C R Balakrishnan, and
F Pirchner
December 2014,
Meta gene,
A Mitra, and
P Schlee, and
I Krause, and
J Blusch, and
T Werner, and
C R Balakrishnan, and
F Pirchner
October 2002,
Journal of dairy science,
A Mitra, and
P Schlee, and
I Krause, and
J Blusch, and
T Werner, and
C R Balakrishnan, and
F Pirchner
January 1987,
Animal genetics,
A Mitra, and
P Schlee, and
I Krause, and
J Blusch, and
T Werner, and
C R Balakrishnan, and
F Pirchner
July 2008,
Genetics and molecular research : GMR,
A Mitra, and
P Schlee, and
I Krause, and
J Blusch, and
T Werner, and
C R Balakrishnan, and
F Pirchner
April 1968,
Comparative biochemistry and physiology,
A Mitra, and
P Schlee, and
I Krause, and
J Blusch, and
T Werner, and
C R Balakrishnan, and
F Pirchner
September 1966,
The Indian veterinary journal,
