-transparent.png){kind=logo}
Cathepsin D, a lysosomal aspartic protease, has been purified from porcine liver using a combination of pepstatin-A agarose and Affi-Gel Blue affinity chromatography, followed by size-exclusion chromatography. The purified protein consists of two polypeptide chains of 15 and 30 kDa, and has an isoelectric point of 6.8. Porcine liver cathepsin D has maximum activity at pH 2.5-3.0 as determined by its activity against hemoglobin, with a Kcat of 14.3 s-1 and a kcat/KM of 2.70 x 10(6) s-1M-1 as determined by the hydrolysis of a fluorogenic peptide substrate.
Related Publications
F Canduri, and
R J Ward, and
W F de Azevedo Júnior, and
R A Gomes, and
R K Arni
September 1985,
Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire,
F Canduri, and
R J Ward, and
W F de Azevedo Júnior, and
R A Gomes, and
R K Arni
June 1993,
Comparative biochemistry and physiology. B, Comparative biochemistry,
F Canduri, and
R J Ward, and
W F de Azevedo Júnior, and
R A Gomes, and
R K Arni
January 1978,
Preparative biochemistry,
F Canduri, and
R J Ward, and
W F de Azevedo Júnior, and
R A Gomes, and
R K Arni
January 1988,
The International journal of biochemistry,
F Canduri, and
R J Ward, and
W F de Azevedo Júnior, and
R A Gomes, and
R K Arni
March 1998,
European journal of biochemistry,
F Canduri, and
R J Ward, and
W F de Azevedo Júnior, and
R A Gomes, and
R K Arni
August 1988,
Biological chemistry Hoppe-Seyler,
F Canduri, and
R J Ward, and
W F de Azevedo Júnior, and
R A Gomes, and
R K Arni
March 2019,
International journal of molecular sciences,
F Canduri, and
R J Ward, and
W F de Azevedo Júnior, and
R A Gomes, and
R K Arni
August 1976,
The Journal of biological chemistry,
F Canduri, and
R J Ward, and
W F de Azevedo Júnior, and
R A Gomes, and
R K Arni
August 1976,
Analytical biochemistry,
F Canduri, and
R J Ward, and
W F de Azevedo Júnior, and
R A Gomes, and
R K Arni
June 2017,
Sheng wu gong cheng xue bao = Chinese journal of biotechnology,
