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Glucose-stimulated expression of the insulin gene in beta cells is mediated by the PDX-1 transcription factor. In this report, we show that stimulation results from effects on activation and DNA-binding potential. Thus, glucose specifically stimulated expression in MIN6 beta cells from chimeras of PDX-1 and the GAL4 DNA-binding domain which spanned the N-terminal PDX-1 activation domain located between amino acids 1 to 79. GAL4:PDX activity was induced over physiological glucose concentrations and was also regulated by effectors of this response. The level of endogenous PDX-1 binding and phosphorylation were also induced under these conditions. We discuss how changes in PDX-1 phosphorylation may influence activity in glucose-treated beta cells.
H V Petersen, and
M Peshavaria, and
A A Pedersen, and
J Philippe, and
R Stein, and
O D Madsen, and
P Serup
October 2008,
Molecules (Basel, Switzerland),
H V Petersen, and
M Peshavaria, and
A A Pedersen, and
J Philippe, and
R Stein, and
O D Madsen, and
P Serup
December 1996,
Biochemical and biophysical research communications,
H V Petersen, and
M Peshavaria, and
A A Pedersen, and
J Philippe, and
R Stein, and
O D Madsen, and
P Serup
November 1999,
FEBS letters,
H V Petersen, and
M Peshavaria, and
A A Pedersen, and
J Philippe, and
R Stein, and
O D Madsen, and
P Serup
March 2002,
The Journal of biological chemistry,
H V Petersen, and
M Peshavaria, and
A A Pedersen, and
J Philippe, and
R Stein, and
O D Madsen, and
P Serup
October 2008,
World journal of gastroenterology,
H V Petersen, and
M Peshavaria, and
A A Pedersen, and
J Philippe, and
R Stein, and
O D Madsen, and
P Serup
August 2011,
The Journal of biological chemistry,
H V Petersen, and
M Peshavaria, and
A A Pedersen, and
J Philippe, and
R Stein, and
O D Madsen, and
P Serup
January 1999,
The Journal of biological chemistry,
H V Petersen, and
M Peshavaria, and
A A Pedersen, and
J Philippe, and
R Stein, and
O D Madsen, and
P Serup
January 2005,
Endocrinology,
H V Petersen, and
M Peshavaria, and
A A Pedersen, and
J Philippe, and
R Stein, and
O D Madsen, and
P Serup
December 1993,
The EMBO journal,
H V Petersen, and
M Peshavaria, and
A A Pedersen, and
J Philippe, and
R Stein, and
O D Madsen, and
P Serup
September 1996,
Proceedings of the National Academy of Sciences of the United States of America,
