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Western blot analysis was used to investigate protein kinase C (PKC) profile of rat eggs. The presence of eight PKC isozymes was demonstrated: conventional PKC alpha, beta and gamma; novel PKC delta, epsilon and mu; atypical PKC zeta and lambda. PKC alpha was detected by RT-PCR as well. PKC translocation from the cytosol to the plasma membrane served as a marker for enzyme activation. Immunofluorescence confocal microscopy demonstrated a relatively uniform distribution of PKC alpha, betaI, and betaII throughout the cytosol of metaphase II arrested eggs. PKC accumulation at the plasma membrane was detected 5 min after exposure to 12-O-tetradecanoyl phorbol-13-acetate and increased with time, thus demonstrating activation of these PKCs.
T Raz, and
E Eliyahu, and
V Yesodi, and
R Shalgi
October 1989,
Gamete research,
T Raz, and
E Eliyahu, and
V Yesodi, and
R Shalgi
July 2002,
Biology of reproduction,
T Raz, and
E Eliyahu, and
V Yesodi, and
R Shalgi
October 1997,
Genes to cells : devoted to molecular & cellular mechanisms,
T Raz, and
E Eliyahu, and
V Yesodi, and
R Shalgi
July 2008,
Proteins,
T Raz, and
E Eliyahu, and
V Yesodi, and
R Shalgi
October 2005,
Reproduction (Cambridge, England),
T Raz, and
E Eliyahu, and
V Yesodi, and
R Shalgi
February 2002,
Molecular and cellular endocrinology,
T Raz, and
E Eliyahu, and
V Yesodi, and
R Shalgi
January 2011,
Theriogenology,
T Raz, and
E Eliyahu, and
V Yesodi, and
R Shalgi
February 1995,
Developmental biology,
T Raz, and
E Eliyahu, and
V Yesodi, and
R Shalgi
April 2004,
Molecular neurobiology,
T Raz, and
E Eliyahu, and
V Yesodi, and
R Shalgi
April 1996,
Current opinion in cell biology,
