Biomaterial Database

A single nonaqueous diazo reagent for the rapid determination of bilirubin in serum without lipemic interference. 1976

L G Morin

A nonaqueous reagent for directly determining serum bilirubin with 3,3'-dimethoxydiphenyl-4,4'-tetrazonium chloride in acidified dimethylsulfoxide is described. Conjugated or unconjugated bilirubin may be determined after extraction in acidified chloroform-ethyl acetate. The procedure is accurate (r2 0.97; recovery 100% for total, 98% for extracted bilirubin) and precise (C.V. 3.0% at 65 mg bilirubin per 1. The reagent develops endpoint color at 590 nm in less than 2 min, and the developed absorbance is stable for several hours. The method is intended for special applications where gross lipemia is encountered. Standardization with pure bilirubin in dimethylsulfoxide or chloroform is identical to standardization with aqueous bilirubin in protein standards.

UI	MeSH Term	Description	Entries
D007202	Indicators and Reagents	Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)	Indicator,Reagent,Reagents,Indicators,Reagents and Indicators
D008055	Lipids	A generic term for fats and lipoids, the alcohol-ether-soluble constituents of protoplasm, which are insoluble in water. They comprise the fats, fatty oils, essential oils, waxes, phospholipids, glycolipids, sulfolipids, aminolipids, chromolipids (lipochromes), and fatty acids. (Grant & Hackh's Chemical Dictionary, 5th ed)	Lipid
D003979	Diazonium Compounds	Azo compounds consisting of an aryl or alkyl group that is joined through two nitrogen atoms to an anion (R-N2+X-).	Compounds, Diazonium
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001663	Bilirubin	A bile pigment that is a degradation product of HEME.	Bilirubin IX alpha,Bilirubin, (15E)-Isomer,Bilirubin, (4E)-Isomer,Bilirubin, (4E,15E)-Isomer,Bilirubin, Calcium Salt,Bilirubin, Disodium Salt,Bilirubin, Monosodium Salt,Calcium Bilirubinate,Hematoidin,delta-Bilirubin,Bilirubinate, Calcium,Calcium Salt Bilirubin,Disodium Salt Bilirubin,Monosodium Salt Bilirubin,Salt Bilirubin, Calcium,delta Bilirubin
D013053	Spectrophotometry	The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.