Growth associated protein 43 (GAP-43) mRNA is upregulated in the rat superior cervical ganglia after preganglionic transection. 1998

X E Hou, and G J Michael, and J V Priestley, and A Dahlström
Department of Anatomy and Cell Biology, Göteborg University Medical Faculty, Sweden. xiu-e.hou@anatcell.gu.se

Growth-associated protein 43 (GAP-43) is a growth-associated protein which is synthesised in high amounts in neurons during neuronal outgrowth. In a previous study we have shown that GAP-43 immunoreactivity is increased in neurons in superior cervical ganglia (SCG) and in nerve terminals in the irides after preganglionic denervation. We have now examined changes in GAP-43 mRNA using in situ hybridisation. GAP-43 mRNA was seen to be constitutively expressed by principal neurons of the rat superior cervical ganglion. Expression was increased further by section of the cervical sympathetic trunk, reaching a maximum (increased by about 30%) 3 days after decentralisation. The increased GAP-43 protein seen after decentralisation thus appears to be due to an upregulation of GAP-43 mRNA in the adrenergic neurons. The results imply that GAP-43 expression in the SCG is under presynaptic control, acting at least partly by control of mRNA levels.

UI MeSH Term Description Entries
D008297 Male Males
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001339 Autonomic Fibers, Preganglionic NERVE FIBERS which project from the central nervous system to AUTONOMIC GANGLIA. In the sympathetic division most preganglionic fibers originate with neurons in the intermediolateral column of the SPINAL CORD, exit via ventral roots from upper thoracic through lower lumbar segments, and project to the paravertebral ganglia; there they either terminate in SYNAPSES or continue through the SPLANCHNIC NERVES to the prevertebral ganglia. In the parasympathetic division the fibers originate in neurons of the BRAIN STEM and sacral spinal cord. In both divisions the principal transmitter is ACETYLCHOLINE but peptide cotransmitters may also be released. Autonomic Fiber, Preganglionic,Fiber, Preganglionic Autonomic,Fibers, Preganglionic Autonomic,Preganglionic Autonomic Fiber,Preganglionic Autonomic Fibers
D012333 RNA, Messenger RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm. Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated
D015854 Up-Regulation A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins. Receptor Up-Regulation,Upregulation,Up-Regulation (Physiology),Up Regulation
D017207 Rats, Sprague-Dawley A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company. Holtzman Rat,Rats, Holtzman,Sprague-Dawley Rat,Rats, Sprague Dawley,Holtzman Rats,Rat, Holtzman,Rat, Sprague-Dawley,Sprague Dawley Rat,Sprague Dawley Rats,Sprague-Dawley Rats
D017403 In Situ Hybridization A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes. Hybridization in Situ,Hybridization, In Situ,Hybridizations, In Situ,In Situ Hybridizations
D017783 Superior Cervical Ganglion The largest and uppermost of the paravertebral sympathetic ganglia. Superior Cervical Ganglia,Cervical Ganglia, Superior,Cervical Ganglion, Superior,Ganglia, Superior Cervical,Ganglion, Superior Cervical
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus
D019922 GAP-43 Protein A nervous tissue specific protein which is highly expressed in NEURONS during development and NERVE REGENERATION. It has been implicated in neurite outgrowth, long-term potentiation, SIGNAL TRANSDUCTION, and NEUROTRANSMITTER release. (From Neurotoxicology 1994;15(1):41-7) It is also a substrate of PROTEIN KINASE C. B-50 Protein,GAP-43,Growth-Associated Protein 43,Nerve Growth Cone Membrane Protein GAP-43,Neuromodulin,GAP43 Protein,Phosphoprotein B-50,Phosphoprotein F1,Phosphoprotein pp46,B 50 Protein,GAP 43 Protein,Growth Associated Protein 43,Nerve Growth Cone Membrane Protein GAP 43,Phosphoprotein B 50

Related Publications

X E Hou, and G J Michael, and J V Priestley, and A Dahlström
March 1997, Neuroreport,
X E Hou, and G J Michael, and J V Priestley, and A Dahlström
May 1993, Brain research. Developmental brain research,
X E Hou, and G J Michael, and J V Priestley, and A Dahlström
February 1984, Bollettino della Societa italiana di biologia sperimentale,
X E Hou, and G J Michael, and J V Priestley, and A Dahlström
January 1991, Restorative neurology and neuroscience,
X E Hou, and G J Michael, and J V Priestley, and A Dahlström
November 1988, Proceedings of the National Academy of Sciences of the United States of America,
X E Hou, and G J Michael, and J V Priestley, and A Dahlström
April 1996, Journal of neuroscience research,
X E Hou, and G J Michael, and J V Priestley, and A Dahlström
February 2006, Endocrinology,
X E Hou, and G J Michael, and J V Priestley, and A Dahlström
December 1995, Neuroreport,
X E Hou, and G J Michael, and J V Priestley, and A Dahlström
September 2013, Neurochemistry international,
X E Hou, and G J Michael, and J V Priestley, and A Dahlström
July 1997, Neuroscience research,
Copied contents to your clipboard!