-transparent.png){kind=logo}
Immunocytological localization of omega-agatoxin IVA (omega-aga IVA)-sensitive Ca2+ channels involved in glutamate release from growth cones of cultured rat dorsal root ganglion (DRG) neurons was studied with field emission scanning electron microscopy (FE-SEM) and transmission electron microscopy (TEM). The omega-aga IVA-sensitive Ca2+ channels were visualized by labeling with immuno-gold particles (30 nm). FE-SEM and TEM images showed that immuno-gold particles were present in the area of growth cones as well as somata, and generally absent on neurite stem and fibroblasts. TEM images of vertical ultra-thin sections showed that the immuno-gold particles were present on the surface of the plasma membrane. Since the gold particles indicate the immunological presence of omega-aga IVA-sensitive Ca2+ channels, the Ca2+ channels involved in transmitter release are present on growth cones before making synapse formation.
H Soeda, and
H Tatsumi, and
Y Kozawa, and
H Mishima, and
Y Katayama
January 1997,
The Journal of membrane biology,
H Soeda, and
H Tatsumi, and
Y Kozawa, and
H Mishima, and
Y Katayama
January 1983,
Nature,
H Soeda, and
H Tatsumi, and
Y Kozawa, and
H Mishima, and
Y Katayama
March 1996,
The European journal of neuroscience,
H Soeda, and
H Tatsumi, and
Y Kozawa, and
H Mishima, and
Y Katayama
September 1997,
Archives italiennes de biologie,
H Soeda, and
H Tatsumi, and
Y Kozawa, and
H Mishima, and
Y Katayama
August 1987,
Pflugers Archiv : European journal of physiology,
H Soeda, and
H Tatsumi, and
Y Kozawa, and
H Mishima, and
Y Katayama
June 1993,
Annals of the New York Academy of Sciences,
H Soeda, and
H Tatsumi, and
Y Kozawa, and
H Mishima, and
Y Katayama
October 2003,
British journal of pharmacology,
H Soeda, and
H Tatsumi, and
Y Kozawa, and
H Mishima, and
Y Katayama
December 1989,
Quarterly journal of experimental physiology (Cambridge, England),
H Soeda, and
H Tatsumi, and
Y Kozawa, and
H Mishima, and
Y Katayama
January 1983,
Annual review of physiology,
H Soeda, and
H Tatsumi, and
Y Kozawa, and
H Mishima, and
Y Katayama
October 1993,
Neuron,
