-transparent.png){kind=logo}
The Sindbis replicon expression system was used to express La Crosse (LAC) virus envelope glycoprotein genes in both mammalian and mosquito cell culture. Replicon expressed LAC proteins had correct molecular mass (Mr) and were antigenically similar to wild type LAC envelope proteins. In addition, LAC G1 and G2 proteins colocalized when expressed from separate constructs in both mammalian and mosquito cells suggesting that they were trafficked through the cell similarly to wild type LAC proteins. A truncated form of the G1 protein was secreted from mosquito cells when expressed alone. The truncated G1 protein was also secreted from mosquito cells when expressed with the G2 protein, but to a lesser extent than when expressed alone, suggesting that the G2 protein sequestered G1 protein intracellularly. The Sindbis replicon system is a powerful tool for the study of LAC virus protein maturation within mosquito cells and mosquitoes.
K I Kamrud, and
K E Olson, and
S Higgs, and
J O Carlson, and
B J Beaty
April 1994,
Virus research,
K I Kamrud, and
K E Olson, and
S Higgs, and
J O Carlson, and
B J Beaty
August 1988,
Virology,
K I Kamrud, and
K E Olson, and
S Higgs, and
J O Carlson, and
B J Beaty
March 2001,
Journal of virology,
K I Kamrud, and
K E Olson, and
S Higgs, and
J O Carlson, and
B J Beaty
March 1999,
Journal of virology,
K I Kamrud, and
K E Olson, and
S Higgs, and
J O Carlson, and
B J Beaty
October 2007,
Virology journal,
K I Kamrud, and
K E Olson, and
S Higgs, and
J O Carlson, and
B J Beaty
April 1985,
Virology,
K I Kamrud, and
K E Olson, and
S Higgs, and
J O Carlson, and
B J Beaty
November 1972,
Journal of virology,
K I Kamrud, and
K E Olson, and
S Higgs, and
J O Carlson, and
B J Beaty
September 1996,
BioTechniques,
K I Kamrud, and
K E Olson, and
S Higgs, and
J O Carlson, and
B J Beaty
February 1993,
The Journal of general virology,
K I Kamrud, and
K E Olson, and
S Higgs, and
J O Carlson, and
B J Beaty
October 1975,
Medical biology,
