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Strong, pliable polyacrylamide gels containing covalently bound phosphate groups have been formed by radical-initiated copolymerization of acrylamide and a phosphorylated, N-substituted derivative of acrylamide. Under conditions of disc electrophoresis, the gels provide enhanced resolution in the separation of hemoglobin A from S, and separate two forms of yeast glucose 6-phosphate dehydrogenase and rabbit muscle glyceraldehyde 3-phosphate dehydrogenase. Preliminary evidence suggests that the improved resolution is due to ionic adsorption of the proteins to immobilized phosphate groups, implying that the gels may function as supports for electrophoretically powered separations involving ion-exchange or affinity chromatography.
E T Harper, and
B H Phelps, and
V F Riser
July 1991,
Clinica chimica acta; international journal of clinical chemistry,
E T Harper, and
B H Phelps, and
V F Riser
January 1974,
Methods in enzymology,
E T Harper, and
B H Phelps, and
V F Riser
September 1982,
Analytical biochemistry,
E T Harper, and
B H Phelps, and
V F Riser
January 2007,
Analytical biochemistry,
E T Harper, and
B H Phelps, and
V F Riser
January 1967,
The New Zealand medical journal,
E T Harper, and
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V F Riser
January 1973,
Molecular biology, biochemistry, and biophysics,
E T Harper, and
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V F Riser
October 1995,
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E T Harper, and
B H Phelps, and
V F Riser
December 1963,
Canadian journal of biochemistry and physiology,
E T Harper, and
B H Phelps, and
V F Riser
June 2006,
CSH protocols,
E T Harper, and
B H Phelps, and
V F Riser
January 1992,
Electrophoresis,
