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A system for automatic analysis of in vitro hematopoietic colonies is described and evaluated. With the standard resolution provided by video cameras, the improvement in visualization obtained using features other than size and darkness when classifying potential colonies appears to be limited. We confirmed this by comparing results obtained with the test system with those obtained with a commercial one. However, for some applications it may be useful to supplement the system with specific methods, e.g., to separate merged colonies. Digital image analyses provide new possibilities, for instance of measuring the total cellularity of the dish or analyzing colonies according to the size and cell density of each colony. Examples provided are time course studies of colony development, cellularity feedback effects on colony sizes, and bell-shaped dose-response curves for the growth stimulation obtained by certain conditioned media on a subpopulation of progenitor cells that gives rise to large colonies.
H B Benestad, and
T Sørensen, and
K M Iranpour, and
K Liestøl, and
K Yogesan, and
I Strøm-Gundersen, and
X Wang, and
D Løvhaug
November 1972,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
H B Benestad, and
T Sørensen, and
K M Iranpour, and
K Liestøl, and
K Yogesan, and
I Strøm-Gundersen, and
X Wang, and
D Løvhaug
January 1995,
Mayo Clinic proceedings,
H B Benestad, and
T Sørensen, and
K M Iranpour, and
K Liestøl, and
K Yogesan, and
I Strøm-Gundersen, and
X Wang, and
D Løvhaug
May 1987,
Experimental hematology,
H B Benestad, and
T Sørensen, and
K M Iranpour, and
K Liestøl, and
K Yogesan, and
I Strøm-Gundersen, and
X Wang, and
D Løvhaug
January 2015,
Journal of microbiological methods,
H B Benestad, and
T Sørensen, and
K M Iranpour, and
K Liestøl, and
K Yogesan, and
I Strøm-Gundersen, and
X Wang, and
D Løvhaug
January 1995,
Dermatology (Basel, Switzerland),
H B Benestad, and
T Sørensen, and
K M Iranpour, and
K Liestøl, and
K Yogesan, and
I Strøm-Gundersen, and
X Wang, and
D Løvhaug
January 1988,
Australasian physical & engineering sciences in medicine,
H B Benestad, and
T Sørensen, and
K M Iranpour, and
K Liestøl, and
K Yogesan, and
I Strøm-Gundersen, and
X Wang, and
D Løvhaug
December 1994,
The Journal of the Greater Houston Dental Society,
H B Benestad, and
T Sørensen, and
K M Iranpour, and
K Liestøl, and
K Yogesan, and
I Strøm-Gundersen, and
X Wang, and
D Løvhaug
January 1989,
Schweizer Monatsschrift fur Zahnmedizin = Revue mensuelle suisse d'odonto-stomatologie = Rivista mensile svizzera di odontologia e stomatologia,
H B Benestad, and
T Sørensen, and
K M Iranpour, and
K Liestøl, and
K Yogesan, and
I Strøm-Gundersen, and
X Wang, and
D Løvhaug
March 1995,
Electrophoresis,
H B Benestad, and
T Sørensen, and
K M Iranpour, and
K Liestøl, and
K Yogesan, and
I Strøm-Gundersen, and
X Wang, and
D Løvhaug
February 2005,
Computer methods and programs in biomedicine,
