BIOMAT Database Logo BIOMAT Database Logo

Estrogenic xenobiotics affect the intracellular activation signal in mitogen-induced human peripheral blood lymphocytes: immunotoxicological impact. 1998

K Sakabe, and M Okuma, and M Kazuno, and T Yamaguchi, and T Yoshida, and H Furuya, and F Kayama, and Y Suwa, and W Fujii, and K L Fresa
Department of Anatomy, Tokai University School of Medicine, Isehara, Kanagawa, Japan. sakbel@ibm.net

The present study was an attempt to elucidate the effect of estrogenic xenobiotics on the proliferation of mitogen-stimulated human peripheral blood lymphocyte (PBL). Our findings follow: (a) the proliferation of PBL in response to phytohemagglutinin (PHA) was mediated by protein kinase C activity, but estrogenic xenobiotics had a strong inhibitory effect on protein kinase C activity of PHA-stimulated PBL; (b) cytoplasmic extracts from PHA-stimulated PBL greatly activated DNA replication, but estrogenic xenobiotics had a strong inhibitory effect on these activities. The results suggest that the cytoplasmic signal-generating system in mitogen-treated PBL is inhibited by estrogenic xenobiotics, and that the defect occurs at all stages in the sequence of events leading to DNA synthesis and cell proliferation.

UI MeSH Term Description Entries
D008213 Lymphocyte Activation Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION. Blast Transformation,Blastogenesis,Lymphoblast Transformation,Lymphocyte Stimulation,Lymphocyte Transformation,Transformation, Blast,Transformation, Lymphoblast,Transformation, Lymphocyte,Activation, Lymphocyte,Stimulation, Lymphocyte
D008214 Lymphocytes White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS. Lymphoid Cells,Cell, Lymphoid,Cells, Lymphoid,Lymphocyte,Lymphoid Cell
D011493 Protein Kinase C An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. The additional presence of DIACYLGLYCEROLS markedly increases its sensitivity to both calcium and phospholipids. The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters. Calcium Phospholipid-Dependent Protein Kinase,Calcium-Activated Phospholipid-Dependent Kinase,PKC Serine-Threonine Kinase,Phospholipid-Sensitive Calcium-Dependent Protein Kinase,Protein Kinase M,Calcium Activated Phospholipid Dependent Kinase,Calcium Phospholipid Dependent Protein Kinase,PKC Serine Threonine Kinase,Phospholipid Sensitive Calcium Dependent Protein Kinase,Phospholipid-Dependent Kinase, Calcium-Activated,Serine-Threonine Kinase, PKC
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D004261 DNA Replication The process by which a DNA molecule is duplicated. Autonomous Replication,Replication, Autonomous,Autonomous Replications,DNA Replications,Replication, DNA,Replications, Autonomous,Replications, DNA
D004958 Estradiol The 17-beta-isomer of estradiol, an aromatized C18 steroid with hydroxyl group at 3-beta- and 17-beta-position. Estradiol-17-beta is the most potent form of mammalian estrogenic steroids. 17 beta-Estradiol,Estradiol-17 beta,Oestradiol,17 beta-Oestradiol,Aerodiol,Delestrogen,Estrace,Estraderm TTS,Estradiol Anhydrous,Estradiol Hemihydrate,Estradiol Hemihydrate, (17 alpha)-Isomer,Estradiol Monohydrate,Estradiol Valerate,Estradiol Valeriante,Estradiol, (+-)-Isomer,Estradiol, (-)-Isomer,Estradiol, (16 alpha,17 alpha)-Isomer,Estradiol, (16 alpha,17 beta)-Isomer,Estradiol, (17-alpha)-Isomer,Estradiol, (8 alpha,17 beta)-(+-)-Isomer,Estradiol, (8 alpha,17 beta)-Isomer,Estradiol, (9 beta,17 alpha)-Isomer,Estradiol, (9 beta,17 beta)-Isomer,Estradiol, Monosodium Salt,Estradiol, Sodium Salt,Estradiol-17 alpha,Estradiol-17beta,Ovocyclin,Progynon-Depot,Progynova,Vivelle,17 beta Estradiol,17 beta Oestradiol,Estradiol 17 alpha,Estradiol 17 beta,Estradiol 17beta,Progynon Depot
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000328 Adult A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available. Adults
D015262 Xenobiotics Chemical substances that are foreign to the biological system. They include naturally occurring compounds, drugs, environmental agents, carcinogens, insecticides, etc. Xenobiotic

Related Publications

K Sakabe, and M Okuma, and M Kazuno, and T Yamaguchi, and T Yoshida, and H Furuya, and F Kayama, and Y Suwa, and W Fujii, and K L Fresa
Redistribution of intracellular Ca2+ in mitogen-stimulated human peripheral blood lymphocytes.
October 1983, General physiology and biophysics,
K Sakabe, and M Okuma, and M Kazuno, and T Yamaguchi, and T Yoshida, and H Furuya, and F Kayama, and Y Suwa, and W Fujii, and K L Fresa
Requirement for extracellular calcium or magnesium in mitogen-induced activation of human peripheral blood lymphocytes.
June 1988, Journal of cellular physiology,
K Sakabe, and M Okuma, and M Kazuno, and T Yamaguchi, and T Yoshida, and H Furuya, and F Kayama, and Y Suwa, and W Fujii, and K L Fresa
Activation of human peripheral blood lymphocytes by a trypanosome-derived mitogen.
September 1981, Clinical and experimental immunology,
K Sakabe, and M Okuma, and M Kazuno, and T Yamaguchi, and T Yoshida, and H Furuya, and F Kayama, and Y Suwa, and W Fujii, and K L Fresa
A sodium requirement for mitogen-induced proliferation in human peripheral blood lymphocytes.
December 1981, Experimental cell research,
K Sakabe, and M Okuma, and M Kazuno, and T Yamaguchi, and T Yoshida, and H Furuya, and F Kayama, and Y Suwa, and W Fujii, and K L Fresa
Protein phosphorylation in human peripheral blood lymphocytes: mitogen-induced increases in protein phosphorylation in intact lymphocytes.
May 1980, Journal of immunology (Baltimore, Md. : 1950),
K Sakabe, and M Okuma, and M Kazuno, and T Yamaguchi, and T Yoshida, and H Furuya, and F Kayama, and Y Suwa, and W Fujii, and K L Fresa
Role of monocytes in pokeweed mitogen-induced differentiation of human peripheral blood lymphocytes.
March 1981, Cellular immunology,
K Sakabe, and M Okuma, and M Kazuno, and T Yamaguchi, and T Yoshida, and H Furuya, and F Kayama, and Y Suwa, and W Fujii, and K L Fresa
The surface morphology of mitogen-stimulated human peripheral blood lymphocytes.
August 1978, British journal of haematology,
K Sakabe, and M Okuma, and M Kazuno, and T Yamaguchi, and T Yoshida, and H Furuya, and F Kayama, and Y Suwa, and W Fujii, and K L Fresa
Transferrin binding by mitogen-activated human peripheral blood lymphocytes.
August 1980, Clinical immunology and immunopathology,
K Sakabe, and M Okuma, and M Kazuno, and T Yamaguchi, and T Yoshida, and H Furuya, and F Kayama, and Y Suwa, and W Fujii, and K L Fresa
Suppression of mitogen-induced proliferation of human peripheral blood lymphocytes by plant lignans.
August 1991, Planta medica,
K Sakabe, and M Okuma, and M Kazuno, and T Yamaguchi, and T Yoshida, and H Furuya, and F Kayama, and Y Suwa, and W Fujii, and K L Fresa
Kinetics of mitogen-induced proliferation and differentiation of human peripheral blood B lymphocytes.
April 1981, Clinical and experimental immunology,
Copied contents to your clipboard!