Biomaterial Database

An intact conformation at the tip of elongation factor G domain IV is functionally important. 1998

K A Martemyanov, and A S Yarunin, and A Liljas, and A T Gudkov

Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region.

Three variants of Thermus thermophilus EF-G with mutations in the loop at the distal end of its domain IV were obtained. The replacement of His-573 by Ala and double mutation H573A/D576A did not influence the functional activity of EF-G. On the other hand, the insertion of six amino acids into the loop between residues Asp-576 and Ser-577 reduced the translocational activity of EF-G markedly, while its GTPase activity was not affected. It is concluded that the native conformation of the loop is important for the factor-promoted translocation in the ribosome. The functional importance of the entire EF-G domain IV is discussed.

UI	MeSH Term	Description	Entries
D009154	Mutation	Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.	Mutations
D010445	Peptide Elongation Factors	Protein factors uniquely required during the elongation phase of protein synthesis.	Elongation Factor,Elongation Factors, Peptide,Factor, Elongation,Factors, Peptide Elongation
D011487	Protein Conformation	The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).	Conformation, Protein,Conformations, Protein,Protein Conformations
D013329	Structure-Activity Relationship	The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.	Relationship, Structure-Activity,Relationships, Structure-Activity,Structure Activity Relationship,Structure-Activity Relationships
D016963	Thermus thermophilus	A species of gram-negative, aerobic, rod-shaped bacteria found in hot springs of neutral to alkaline pH, as well as in hot-water heaters.
D020653	Peptide Elongation Factor G	Peptide Elongation Factor G catalyzes the translocation of peptidyl-tRNA from the A to the P site of bacterial ribosomes by a process linked to hydrolysis of GTP to GDP.	EF-G,Elongation Factor G,fusA Gene Product,fusA Protein,EF G
D020963	GTP Phosphohydrolase-Linked Elongation Factors	Factors that utilize energy from the hydrolysis of GTP to GDP for peptide chain elongation. EC 3.6.1.-.	GTPase-Linked Elongation Factors,Guanosinetriphosphatase-Linked Elongation Factors,Elongation Factors, GTPase-Linked,Elongation Factors, Guanosinetriphosphatase-Linked,Factors, GTPase-Linked Elongation,Factors, Guanosinetriphosphatase-Linked Elongation,GTP Phosphohydrolase Linked Elongation Factors,GTPase Linked Elongation Factors,Guanosinetriphosphatase Linked Elongation Factors