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A fluorescent probe N-(3-pyrene)maleimide was conjugated to rabbit skeletal F-actin at the site of most reactive sulfhydryl group (Cys-373). Its fluorescence anisotropy decay showed a single correlation time of 560 ns at 25 degrees C, which is in a very good agreement with the correlation time of the dansyl-L-cysteine group conjugated to the same site of F-actin reported very recently [Wahl, Ph., Mihashi, K, and Auchet, J-C. (1975) FEBS Lett. 8, 164-167]. Actin from plasmodia of myxomycates, Physarum polycepharum, was also conjugated with N-(3-pyrene) maleimide and the fluorescence anisotropy was compared with rabbit skeletal F-actin using the classical steady excitation method. It was found that the internal mobility of the magnesium polymer of plasmodium actin is remarkably larger than both plasmodium F-actin and rabbit skeletal F-actin.
Y Kawasaki, and
K Mihashi, and
H Tanaka, and
H Ohnuma
April 1980,
European journal of biochemistry,
Y Kawasaki, and
K Mihashi, and
H Tanaka, and
H Ohnuma
June 1978,
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Y Kawasaki, and
K Mihashi, and
H Tanaka, and
H Ohnuma
January 1979,
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Y Kawasaki, and
K Mihashi, and
H Tanaka, and
H Ohnuma
May 1989,
Current eye research,
Y Kawasaki, and
K Mihashi, and
H Tanaka, and
H Ohnuma
May 1973,
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Y Kawasaki, and
K Mihashi, and
H Tanaka, and
H Ohnuma
March 1992,
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Y Kawasaki, and
K Mihashi, and
H Tanaka, and
H Ohnuma
January 1976,
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Y Kawasaki, and
K Mihashi, and
H Tanaka, and
H Ohnuma
June 1976,
European journal of biochemistry,
Y Kawasaki, and
K Mihashi, and
H Tanaka, and
H Ohnuma
September 1978,
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Y Kawasaki, and
K Mihashi, and
H Tanaka, and
H Ohnuma
May 2007,
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