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[Analysis of transmission of Burkholderia cepacia isolates in an intrahospital by randomly amplified polymorphic DNA-PCR method]. 1998

M Okazaki, and K Morita, and N Kochi, and K Araki, and M Yoshizawa, and H Wada, and M Shibata, and N Watanabe, and T Egami, and N Furuya, and M Kanamori, and S Shimazaki, and H Uchimura
Department of Clinical Laboratories, Kyorin University Hospital.

Strains of Burkholderia cepacia isolated in our hospital from November 1995 to September 1996 were classified with randomly amplified polymorphic DNA-PCR (RAPD-PCR) and conventional biochemical tests (ID test.NF-18, API20NE, and Neg Combo 4J kit), and intrahospital isolates of B. cepacia were analysed. During the period 28 strains from inpatients and 2 from medical apparatus were isolated. Twenty four of 28 (85.7%) were from sputum. In 1996 from January to February, 20 strains were detected from 8 inpatients, and two strains were from the nebulizers at the Trauma and Critical Care Center (TCC). With typing of B. cepacia by conventional methods no epidemiological relations among isolates were found. However, DNA patterns of original isolates from the nebulizers at TCC by RAPD-PCR were identical with those of isolates in sputa from patients in other wards who had stayed at TCC, indicating that TCC was an initial source of transmission and the strain was transmitted with the patients to the wards. These results suggest that RAPD-PCR method might be a useful tool to analyse an epidemiological survey for intrahospital transmission of isolate.

UI MeSH Term Description Entries
D003428 Cross Infection Any infection which a patient contracts in a health-care institution. Hospital Infections,Nosocomial Infections,Health Care Associated Infection,Health Care Associated Infections,Healthcare Associated Infections,Infection, Cross,Infections, Hospital,Infections, Nosocomial,Cross Infections,Healthcare Associated Infection,Hospital Infection,Infection, Healthcare Associated,Infection, Hospital,Infection, Nosocomial,Infections, Cross,Infections, Healthcare Associated,Nosocomial Infection
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D016956 Burkholderia cepacia A species of BURKHOLDERIA considered to be an opportunistic human pathogen. It has been associated with various types of infections of nosocomial origin. Pseudomonas cepacia,Pseudomonas kingii,Pseudomonas multivorans
D019105 Random Amplified Polymorphic DNA Technique Technique that utilizes low-stringency polymerase chain reaction (PCR) amplification with single primers of arbitrary sequence to generate strain-specific arrays of anonymous DNA fragments. RAPD technique may be used to determine taxonomic identity, assess kinship relationships, analyze mixed genome samples, and create specific probes. RAPD Technique,RAPD-PCR,Arbitrarily Primed PCR Reaction,RAPD Technic,Random Amplified Polymorphic DNA Technic,RAPD Technics,RAPD Techniques
D019121 Burkholderia Infections Infections with bacteria of the genus BURKHOLDERIA. B cepacia Infection,B. cepacia Infection,Burkholderia cepacia Infection,Burkholderia cepacia Sepsis,Infections, Burkholderia,B cepacia Infections,B. cepacia Infections,Burkholderia Infection,Burkholderia cepacia Infections,Burkholderia cepacia Sepses,Infection, B cepacia,Infection, B. cepacia,Infection, Burkholderia,Infection, Burkholderia cepacia,Infections, B cepacia,Sepses, Burkholderia cepacia,Sepsis, Burkholderia cepacia

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