Biomaterial Database

Physical characteristics of DNA from bacteriophages of Agrobacterium tumefaciens. 1976

R J Manasse, and R C Staples

DNA was extracted from isolates of bacteriophages grown on virulent and avirulent strains of Agrobacterium tumefaciens. Molecular weights of DNA from phages isolated from the virulent A. tumefaciens (IIBV7) were about 41.5 X 10(6) daltons, while those from the avirulent A. tumefaciens (IIBNV6) were about 32.5 X 10(6) daltons. The buoyant densities of the four DNA's ranged from 1.7086 to 1.7089 g/cm3, values that were not significantly different. DNA-DNA hybridization studies also indicated that the four phages were closely related. Attempts to induce tumors with phage DNA were unsuccessful.

UI	MeSH Term	Description	Entries
D008970	Molecular Weight	The sum of the weight of all the atoms in a molecule.	Molecular Weights,Weight, Molecular,Weights, Molecular
D009690	Nucleic Acid Conformation	The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.	DNA Conformation,RNA Conformation,Conformation, DNA,Conformation, Nucleic Acid,Conformation, RNA,Conformations, DNA,Conformations, Nucleic Acid,Conformations, RNA,DNA Conformations,Nucleic Acid Conformations,RNA Conformations
D010941	Plant Tumors	A localized proliferation of plant tissue forming a swelling or outgrowth, commonly with a characteristic shape and unlike any organ of the normal plant. Plant tumors or galls usually form in response to the action of a pathogen or a pest. (Holliday, P., A Dictionary of Plant Pathology, 1989, p330)	Crown Gall,Galls, Plant,Plant Galls,Crown Galls,Gall, Crown,Gall, Plant,Galls, Crown,Plant Gall,Plant Tumor,Tumor, Plant,Tumors, Plant
D004279	DNA, Viral	Deoxyribonucleic acid that makes up the genetic material of viruses.	Viral DNA
D001435	Bacteriophages	Viruses whose hosts are bacterial cells.	Phages,Bacteriophage,Phage
D012231	Rhizobium	A genus of gram-negative, aerobic, rod-shaped bacteria that activate PLANT ROOT NODULATION in leguminous plants. Members of this genus are nitrogen-fixing and common soil inhabitants.
D014774	Virulence	The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.	Pathogenicity