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Tissue culture assays using Caco-2 cell line differentiate virulent from non-virulent Listeria monocytogenes strains. 1998

N Van Langendonck, and E Bottreau, and S Bailly, and M Tabouret, and J Marly, and P Pardon, and P Velge
Institut National de la Recherche Agronomique de tours, Laboratoire de Pathologie Infectieuse et Immunologie, Nouzilly, France.

Within the group of Listeria sp., only L. monocytogenes is pathogenic for humans and numerous studies of L. monocytogenes strains have described non-virulent isolates. In this study, the potential value of two tissue culture assays (TCA) was analysed to ascertain the virulence properties of L. monocytogenes strains, initially typed for virulence using the immunocompromised mouse model (ICMM). The first assay assessed both the penetration into, and multiplication within, Caco-2 cells (PM assay): the second was a plaque-forming assay (PF assay). All the clinical isolates (nine strains) were virulent in both TCA. Conversely, all the non-pathogenic species (seven strains) were non-virulent in PM and PF assays. Compared with the virulence obtained in the ICMM with 29 Listeria strains, including 12 non-virulent L. monocytogenes strains, the sensitivity of both TCA was equal to1. Specificity was 0.89 and 0.84 for the PF and PM assays, respectively. However, a study of strains exhibiting virulence differences in three other in vivo virulence models showed that ICMM only detected highly virulent strains. The specificity of the PF test could, therefore, be higher, and close to that obtained by the enumeration of viable bacteria in the spleen of mice infected by subcutaneous injection in the footpad and by intravenous injection. Taken together, this study confirms the existence of low-virulence L. monocytogenes strains and shows that the virulence status of some non-clinical L. monocytogenes isolates depends on the virulence models used. The data suggest that the PF assay could be used as a primary test to evaluate the virulence of Listeria strains in order to reduce the cost of testing all strains in vivo.

UI MeSH Term Description Entries
D007121 Immunocompetence The ability of lymphoid cells to mount a humoral or cellular immune response when challenged by antigen. Competence, Immunologic,Immunologic Competence,Immunological Competence,Competence, Immunological
D007700 Kinetics The rate dynamics in chemical or physical systems.
D008089 Listeria monocytogenes A species of gram-positive, rod-shaped bacteria widely distributed in nature. It has been isolated from sewage, soil, silage, and from feces of healthy animals and man. Infection with this bacterium leads to encephalitis, meningitis, endocarditis, and abortion.
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D014407 Tumor Cells, Cultured Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely. Cultured Tumor Cells,Neoplastic Cells, Cultured,Cultured Neoplastic Cells,Cell, Cultured Neoplastic,Cell, Cultured Tumor,Cells, Cultured Neoplastic,Cells, Cultured Tumor,Cultured Neoplastic Cell,Cultured Tumor Cell,Neoplastic Cell, Cultured,Tumor Cell, Cultured
D014774 Virulence The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS. Pathogenicity
D016867 Immunocompromised Host A human or animal whose immunologic mechanism is deficient because of an immunodeficiency disorder or other disease or as the result of the administration of immunosuppressive drugs or radiation. Immunosuppressed Host,Immunocompromised Patient,Host, Immunocompromised,Host, Immunosuppressed,Hosts, Immunocompromised,Hosts, Immunosuppressed,Immunocompromised Hosts,Immunocompromised Patients,Immunosuppressed Hosts,Patient, Immunocompromised,Patients, Immunocompromised
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D018938 Caco-2 Cells Human colonic ADENOCARCINOMA cells that are able to express differentiation features characteristic of mature intestinal cells, such as ENTEROCYTES. These cells are valuable in vitro tools for studies related to intestinal cell function and differentiation. Caco 2 Cells,Caco-2 Cell,Cell, Caco-2,Cells, Caco-2

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