Biomaterial Database

Physical mapping of the conjugative plasmid pMB1-1 of Enterococcus faecalis. 1998

M Martínez-Bueno, and E Valdivia, and I Martín, and R Quirantes, and M Maqueda

Departamento de Microbiología, Facultad Ciencias, University of Granada, Spain.

The sexpheromone system of Enterococcus faecalis is a form of bacterial conjugation that plays an important role in the horizontal dissemination of genes. The ecological significance of 'sexual' plasmids is to permit a rapid mobilization of genes of interest for the species (e.g. those encoding haemolysins, bacteriocins or antibiotic resistance). The physical mapping of pMB1-1, a conjugative plasmid of Ent. faecalis that responds to cCF10 pheromone, has been undertaken. By means of hybridization with conserved sequences of pCF10 plasmid, the regions harbouring the genes responsible for the pheromone inhibitor and the aggregation and exclusion proteins of this plasmid have been identified. The results demonstrated that plasmids pMB1-1 and pCF10 only show homology in the region involved in the conjugative response, suggesting that this region may be transferred in an independent way to that of the rest of the plasmid.

UI	MeSH Term	Description	Entries
D009842	Oligopeptides	Peptides composed of between two and twelve amino acids.	Oligopeptide
D010675	Pheromones	Chemical substances, excreted by an organism into the environment, that elicit behavioral or physiological responses from other organisms of the same species. Perception of these chemical signals may be olfactory or by contact.	Allelochemical,Allelochemicals,Allomone,Allomones,Ectohormones,Kairomone,Kairomones,Pheromone,Semiochemical,Semiochemicals,Synomones
D010957	Plasmids	Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.	Episomes,Episome,Plasmid
D003227	Conjugation, Genetic	A parasexual process in BACTERIA; ALGAE; FUNGI; and ciliate EUKARYOTA for achieving exchange of chromosome material during fusion of two cells. In bacteria, this is a uni-directional transfer of genetic material; in protozoa it is a bi-directional exchange. In algae and fungi, it is a form of sexual reproduction, with the union of male and female gametes.	Bacterial Conjugation,Conjugation, Bacterial,Genetic Conjugation
D004269	DNA, Bacterial	Deoxyribonucleic acid that makes up the genetic material of bacteria.	Bacterial DNA
D013293	Enterococcus faecalis	A species of gram-positive, coccoid bacteria commonly isolated from clinical specimens and the human intestinal tract. Most strains are nonhemolytic.	Streptococcus Group D,Streptococcus faecalis
D015139	Blotting, Southern	A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.	Southern Blotting,Blot, Southern,Southern Blot
D015183	Restriction Mapping	Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.	Endonuclease Mapping, Restriction,Enzyme Mapping, Restriction,Site Mapping, Restriction,Analysis, Restriction Enzyme,Enzyme Analysis, Restriction,Restriction Enzyme Analysis,Analyses, Restriction Enzyme,Endonuclease Mappings, Restriction,Enzyme Analyses, Restriction,Enzyme Mappings, Restriction,Mapping, Restriction,Mapping, Restriction Endonuclease,Mapping, Restriction Enzyme,Mapping, Restriction Site,Mappings, Restriction,Mappings, Restriction Endonuclease,Mappings, Restriction Enzyme,Mappings, Restriction Site,Restriction Endonuclease Mapping,Restriction Endonuclease Mappings,Restriction Enzyme Analyses,Restriction Enzyme Mapping,Restriction Enzyme Mappings,Restriction Mappings,Restriction Site Mapping,Restriction Site Mappings,Site Mappings, Restriction