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Histone gene expression was studied during the cell cycle of continuously dividing HeLa S3-cells and following stimulation of confluent monolayers of WI-38 human diploid fibroblasts to proliferate. The presence of histone messenger RNA (mRNA) sequences was assayed by hybridization to a 3H-labeled single-stranded DNA complementary to histone mRNA's. In HeLa S3-cells, histone mRNA sequences were found in the nucleus and associated with polyribosomes during S phase but not during G1. Transcripts of S-phase chromatin contained histone mRNA sequences but those of G1 chromatin did not. Similarly, in WI-38 cells association of histone mRNA sequences with polyribosomes and transcription of histone mRNA sequences from chromatin parallel DNA replication. Together these results suggest that the regulation of histone gene expression resides, at least in part, at the transcriptional level. Chromatin reconstitution studies provide evidence that nonhistone chromosomal proteins play a key role in activation of histone gene transcription during the period of the cell cycle when DNA is replicated. Phosphate groups associated with the S-phase nonhistone chromosomal proteins appear to be functionally involved in the control of histone gene readout. Although WI-38 human diploid fibroblasts transformed by SV40 exhibit morphological and biochemical modifications characteristic of neoplastic cells, transcription of histone mRNA sequences remains unaltered.
G S Stein, and
J L Stein, and
L J Kleinsmith, and
J A Thomson, and
W D Park, and
R L Jansing
January 1978,
Contemporary topics in molecular immunology,
G S Stein, and
J L Stein, and
L J Kleinsmith, and
J A Thomson, and
W D Park, and
R L Jansing
January 1982,
CRC critical reviews in biochemistry,
G S Stein, and
J L Stein, and
L J Kleinsmith, and
J A Thomson, and
W D Park, and
R L Jansing
March 1980,
Sheng li ke xue jin zhan [Progress in physiology],
G S Stein, and
J L Stein, and
L J Kleinsmith, and
J A Thomson, and
W D Park, and
R L Jansing
September 1994,
FASEB journal : official publication of the Federation of American Societies for Experimental Biology,
G S Stein, and
J L Stein, and
L J Kleinsmith, and
J A Thomson, and
W D Park, and
R L Jansing
February 1984,
Biochimica et biophysica acta,
G S Stein, and
J L Stein, and
L J Kleinsmith, and
J A Thomson, and
W D Park, and
R L Jansing
January 1977,
Acta biologica et medica Germanica,
G S Stein, and
J L Stein, and
L J Kleinsmith, and
J A Thomson, and
W D Park, and
R L Jansing
August 1981,
Biochemistry,
G S Stein, and
J L Stein, and
L J Kleinsmith, and
J A Thomson, and
W D Park, and
R L Jansing
January 2013,
PloS one,
G S Stein, and
J L Stein, and
L J Kleinsmith, and
J A Thomson, and
W D Park, and
R L Jansing
January 1978,
Methods in cell biology,
G S Stein, and
J L Stein, and
L J Kleinsmith, and
J A Thomson, and
W D Park, and
R L Jansing
January 1978,
Methods in cell biology,
