Identification and characterization of a novel member of the fibroblast growth factor family. 1998

J M Greene, and Y L Li, and P A Yourey, and J Gruber, and K C Carter, and B K Shell, and P A Dillon, and C Florence, and D R Duan, and A Blunt, and D M Ornitz, and S M Ruben, and R F Alderson
Department of Molecular Biology and Pharmacology, Washington University, School of Medicine, St. Louis, Missouri 63110, USA.

A new member of the fibroblast growth factor (FGF) family, FGF-13, has been molecularly cloned as a result of high throughput sequencing of a human ovarian cancer cell library. The open reading frame of the novel human gene (1419 bp) encodes for a protein of 216 a.a. with a molecular weight of 22 kDa. The FGF-13 sequence contains an amino-terminal hydrophobic region of 23 a.a. characteristic of a signal secretion sequence. FGF-13 is most homologous, 70% similarity at the amino acid level, to FGF-8. Northern hybridization analysis demonstrated prominent expression of FGF-13 in human foetal and adult brain, particularly in the cerebellum and cortex. In proliferation studies with BaF3 cells, FGF-13 preferentially activates cell clones expressing either FGF receptor variant, 3-IIIc or 4. The signal transduction pathways of FGF-13 and FGF-2 were compared in rat hippocampal astrocytes. The two FGFs induce an equivalent level of tyrosine phosphorylation of mitogen-activated protein kinase (MAPK) and c-raf activation. However, FGF-13 is more effective than FGF-2 in inducing the phosphorylation of phospholipase C-gamma (PLC-gamma). Treatment of neuronal cultures from rat embryonic cortex with FGF-13 increases the number of glutamic acid decarboxylase immunopositive neurons, the level of high-affinity gamma-aminobutyric acid (GABA) uptake, and choline acetyltransferase enzyme activity. The GABAergic neuronal response to FGF-13 treatment is rapid with a significant increase occurring within 72 h. We have identified a novel member of the FGF family that is expressed in the central nervous system (CNS) and increases the number as well as the level of phenotypic differentiation of cortical neurons in vitro.

UI MeSH Term Description Entries
D007668 Kidney Body organ that filters blood for the secretion of URINE and that regulates ion concentrations. Kidneys
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009474 Neurons The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM. Nerve Cells,Cell, Nerve,Cells, Nerve,Nerve Cell,Neuron
D010641 Phenotype The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment. Phenotypes
D011994 Recombinant Proteins Proteins prepared by recombinant DNA technology. Biosynthetic Protein,Biosynthetic Proteins,DNA Recombinant Proteins,Recombinant Protein,Proteins, Biosynthetic,Proteins, Recombinant DNA,DNA Proteins, Recombinant,Protein, Biosynthetic,Protein, Recombinant,Proteins, DNA Recombinant,Proteins, Recombinant,Recombinant DNA Proteins,Recombinant Proteins, DNA
D001921 Brain The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM. Encephalon
D002454 Cell Differentiation Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs. Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D005346 Fibroblast Growth Factors A family of small polypeptide growth factors that share several common features including a strong affinity for HEPARIN, and a central barrel-shaped core region of 140 amino acids that is highly homologous between family members. Although originally studied as proteins that stimulate the growth of fibroblasts this distinction is no longer a requirement for membership in the fibroblast growth factor family. DNA Synthesis Factor,Fibroblast Growth Factor,Fibroblast Growth Regulatory Factor,Growth Factor, Fibroblast,Growth Factors, Fibroblast
D005810 Multigene Family A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed) Gene Clusters,Genes, Reiterated,Cluster, Gene,Clusters, Gene,Families, Multigene,Family, Multigene,Gene Cluster,Gene, Reiterated,Multigene Families,Reiterated Gene,Reiterated Genes

Related Publications

J M Greene, and Y L Li, and P A Yourey, and J Gruber, and K C Carter, and B K Shell, and P A Dillon, and C Florence, and D R Duan, and A Blunt, and D M Ornitz, and S M Ruben, and R F Alderson
January 1990, Journal of cell science. Supplement,
J M Greene, and Y L Li, and P A Yourey, and J Gruber, and K C Carter, and B K Shell, and P A Dillon, and C Florence, and D R Duan, and A Blunt, and D M Ornitz, and S M Ruben, and R F Alderson
March 1990, Nature,
J M Greene, and Y L Li, and P A Yourey, and J Gruber, and K C Carter, and B K Shell, and P A Dillon, and C Florence, and D R Duan, and A Blunt, and D M Ornitz, and S M Ruben, and R F Alderson
February 1998, Biochemical and biophysical research communications,
J M Greene, and Y L Li, and P A Yourey, and J Gruber, and K C Carter, and B K Shell, and P A Dillon, and C Florence, and D R Duan, and A Blunt, and D M Ornitz, and S M Ruben, and R F Alderson
October 1998, Molecular and cellular biology,
J M Greene, and Y L Li, and P A Yourey, and J Gruber, and K C Carter, and B K Shell, and P A Dillon, and C Florence, and D R Duan, and A Blunt, and D M Ornitz, and S M Ruben, and R F Alderson
July 1996, The Journal of biological chemistry,
J M Greene, and Y L Li, and P A Yourey, and J Gruber, and K C Carter, and B K Shell, and P A Dillon, and C Florence, and D R Duan, and A Blunt, and D M Ornitz, and S M Ruben, and R F Alderson
January 1991, Annals of the New York Academy of Sciences,
J M Greene, and Y L Li, and P A Yourey, and J Gruber, and K C Carter, and B K Shell, and P A Dillon, and C Florence, and D R Duan, and A Blunt, and D M Ornitz, and S M Ruben, and R F Alderson
November 2014, Amino acids,
J M Greene, and Y L Li, and P A Yourey, and J Gruber, and K C Carter, and B K Shell, and P A Dillon, and C Florence, and D R Duan, and A Blunt, and D M Ornitz, and S M Ruben, and R F Alderson
March 1989, Oncogene,
J M Greene, and Y L Li, and P A Yourey, and J Gruber, and K C Carter, and B K Shell, and P A Dillon, and C Florence, and D R Duan, and A Blunt, and D M Ornitz, and S M Ruben, and R F Alderson
June 1991, Oncogene,
J M Greene, and Y L Li, and P A Yourey, and J Gruber, and K C Carter, and B K Shell, and P A Dillon, and C Florence, and D R Duan, and A Blunt, and D M Ornitz, and S M Ruben, and R F Alderson
January 1991, Oncogene,
Copied contents to your clipboard!