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Heparin, an anticoagulant, has been shown to reduce neointimal proliferation and restenosis following vascular injury in experimental studies, but the clinical trials of heparin in coronary balloon angioplasty have been negative. The current study, therefore, examined the effect of heparin on basal or stimulated migration by serum and platelet-derived growth factor (PDGF)-BB in cultured human coronary artery smooth muscle cells (SMCs) by Boyden's chamber method. In addition, the reversibility of the heparin effect on human coronary artery SMC migration was examined. Fetal calf serum (FCS) and PDGF-BB stimulated SMC migration in a concentration-dependent manner. Heparin in moderate to high concentration (10 to 100 U/mL) exhibited concentration-related inhibition of FCS- and PDGF-BB-stimulated SMC migration; however, a low concentration (1 U/mL) of heparin had no inhibitory effects. Heparin also had weak inhibitory effects on nonstimulated SMC migration. The SMCs that were exposed to a high concentration (100 U/mL) of heparin for 6 hours were capable of migrating after a short lag period of removal of heparin from the culture medium. These SMCs also showed recovery of responses to FCS and PDGF-BB by migrating significantly greater than the nonstimulated level. Furthermore, heparin-containing medium did not contain detached cells. These results indicate that heparin inhibits human coronary artery SMC migration, especially when stimulated by FCS or PDGF-BB, and that this inhibitory effect of heparin is reversible and not simply a function of killing cells.
M Kohno, and
K Yokokawa, and
K Yasunari, and
M Minami, and
H Kano, and
A K Mandal, and
J Yoshikawa
January 2012,
PloS one,
M Kohno, and
K Yokokawa, and
K Yasunari, and
M Minami, and
H Kano, and
A K Mandal, and
J Yoshikawa
December 2000,
Journal of molecular and cellular cardiology,
M Kohno, and
K Yokokawa, and
K Yasunari, and
M Minami, and
H Kano, and
A K Mandal, and
J Yoshikawa
February 2012,
Journal of cardiovascular pharmacology,
M Kohno, and
K Yokokawa, and
K Yasunari, and
M Minami, and
H Kano, and
A K Mandal, and
J Yoshikawa
September 1999,
Arteriosclerosis, thrombosis, and vascular biology,
M Kohno, and
K Yokokawa, and
K Yasunari, and
M Minami, and
H Kano, and
A K Mandal, and
J Yoshikawa
November 2012,
Journal of thrombosis and haemostasis : JTH,
M Kohno, and
K Yokokawa, and
K Yasunari, and
M Minami, and
H Kano, and
A K Mandal, and
J Yoshikawa
July 1993,
British journal of pharmacology,
M Kohno, and
K Yokokawa, and
K Yasunari, and
M Minami, and
H Kano, and
A K Mandal, and
J Yoshikawa
June 2015,
Journal of the renin-angiotensin-aldosterone system : JRAAS,
M Kohno, and
K Yokokawa, and
K Yasunari, and
M Minami, and
H Kano, and
A K Mandal, and
J Yoshikawa
February 2002,
Surgery,
M Kohno, and
K Yokokawa, and
K Yasunari, and
M Minami, and
H Kano, and
A K Mandal, and
J Yoshikawa
November 1996,
The Journal of clinical investigation,
M Kohno, and
K Yokokawa, and
K Yasunari, and
M Minami, and
H Kano, and
A K Mandal, and
J Yoshikawa
November 2018,
Experimental and therapeutic medicine,
