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The DNA synthesis pattern and several kinetic parameters of in vitro PHA stimulated normal and CLL lymphocytes were determined. The DNA synthesis peak of CLL lymphocytes occurred 2-3 days later than that of normal lymphocytes. The generation time, estimated by the labeled mitoses method, was found to be 28 hr and 20 hr for CLL and normal lymphocytes respectively. This difference was mainly due to longer S and G1 periods. It was also shown that both CLL and normal lymphocytes divide several times. These data were confirmed by the chromatid labeling pattern and by the halving of the grains and the double labeling techniques. By combining continuous and pulse labeling the growth fraction of CLL lymphocytes was found to be progressively increasing, because of the recruitment of new cells in cycle, from the third day of culture. Therefore the delayed peak of DNA synthesis of CLL lymphocytes was caused by a longer cell cycle and by a longer pre-replicative phase.
B Jaksic, and
G L Pagliardi, and
L Pegoraro
April 1968,
Nihon Naika Gakkai zasshi. The Journal of the Japanese Society of Internal Medicine,
B Jaksic, and
G L Pagliardi, and
L Pegoraro
January 1967,
Archives of pathology,
B Jaksic, and
G L Pagliardi, and
L Pegoraro
August 1969,
Blood,
B Jaksic, and
G L Pagliardi, and
L Pegoraro
January 1973,
Acta haematologica,
B Jaksic, and
G L Pagliardi, and
L Pegoraro
July 1976,
Journal of the National Cancer Institute,
B Jaksic, and
G L Pagliardi, and
L Pegoraro
December 1969,
Biochimica et biophysica acta,
B Jaksic, and
G L Pagliardi, and
L Pegoraro
January 1982,
Comptes rendus des seances de la Societe de biologie et de ses filiales,
B Jaksic, and
G L Pagliardi, and
L Pegoraro
April 1984,
Cancer genetics and cytogenetics,
B Jaksic, and
G L Pagliardi, and
L Pegoraro
October 1979,
Experimental cell research,
B Jaksic, and
G L Pagliardi, and
L Pegoraro
March 1968,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
