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The results of a conformational study by nuclear magnetic spectroscopy and computational methods on a series of point-mutated synthetic peptides, containing 14 amino acid residues and mimicking the region containing the Arg-Lys dibasic cleavage site of pro-somatostatin, have confirmed the possible role of a well defined secondary structure in the recognition phenomenon by processing enzymes. The importance of the residues located near the Arg-Lys dibasic site in the C-terminal region of the pro-hormone for the cleavage of the precursor into somatostatin-14 has been confirmed. The present structural analysis indicates the occurrence of two beta-turns in the 4-7 and 11-14 regions, flanking the cleavage site, for all the peptides recognized as substrates by the processing enzyme. Interestingly, in the point-mutated analogue not processed by the enzyme and containing the replacement of proline by alanine in position 5 the first -turn is displaced by one residue and involves the Ala5-Arg8 segment. This observation may explain the lack of recognition by the maturation enzyme.
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F Fraternali, and
D M Manduca, and
G D'Auria, and
M Simonetti, and
C Di Bello, and
L Paolillo
May 2003,
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D M Manduca, and
G D'Auria, and
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C Di Bello, and
L Paolillo
June 1999,
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L Falcigno, and
F Fraternali, and
D M Manduca, and
G D'Auria, and
M Simonetti, and
C Di Bello, and
L Paolillo
June 1999,
The Journal of organic chemistry,
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D M Manduca, and
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M Simonetti, and
C Di Bello, and
L Paolillo
February 2006,
Journal of peptide science : an official publication of the European Peptide Society,
L Falcigno, and
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L Paolillo
November 1989,
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L Falcigno, and
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L Paolillo
January 1990,
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L Falcigno, and
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C Di Bello, and
L Paolillo
March 2013,
The journal of physical chemistry. B,
L Falcigno, and
F Fraternali, and
D M Manduca, and
G D'Auria, and
M Simonetti, and
C Di Bello, and
L Paolillo
January 1986,
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L Falcigno, and
F Fraternali, and
D M Manduca, and
G D'Auria, and
M Simonetti, and
C Di Bello, and
L Paolillo
July 1997,
The journal of peptide research : official journal of the American Peptide Society,
L Falcigno, and
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G D'Auria, and
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L Paolillo
October 1987,
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