A precision machine for mounting tissue for Mohs micrographic surgery. 1998

J W Franks

BACKGROUND Mounting specimens onto chucks, so the cryostat knife follows the exact path of the surgeon's knife, poses difficulties even for experienced histotechnologists. Oftentimes, the frozen planar surface of the tissue falls at an angle that requires gimballing the chuck holder to compensate. OBJECTIVE To demonstrate the use of a precision tissue mounting machine and associated fast freezing chucks. METHODS Specimens of pickled pig skin were marked with blue dye on the cut surface. The pieces were mounted onto chucks with the blue surface exposed, and then sectioned in a cryostat. RESULTS A complete section with all edges was obtained after eight turns of the cryostat handwheel, at 12 microns per turn. CONCLUSIONS A precision machine used in conjunction with a properly adjusted chuck holder can yield results unobtainable by other methods.

UI MeSH Term Description Entries
D004867 Equipment Design Methods and patterns of fabricating machines and related hardware. Design, Equipment,Device Design,Medical Device Design,Design, Medical Device,Designs, Medical Device,Device Design, Medical,Device Designs, Medical,Medical Device Designs,Design, Device,Designs, Device,Designs, Equipment,Device Designs,Equipment Designs
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013048 Specimen Handling Procedures for collecting, preserving, and transporting of specimens sufficiently stable to provide accurate and precise results suitable for clinical interpretation. Specimen Collection,Collection, Specimen,Collections, Specimen,Handling, Specimen,Handlings, Specimen,Specimen Collections,Specimen Handlings
D013552 Swine Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA). Phacochoerus,Pigs,Suidae,Warthogs,Wart Hogs,Hog, Wart,Hogs, Wart,Wart Hog
D015580 Mohs Surgery A surgical technique used primarily in the treatment of skin neoplasms, especially basal cell or squamous cell carcinoma of the skin. This procedure is a microscopically controlled excision of cutaneous tumors either after fixation in vivo or after freezing the tissue. Serial examinations of fresh tissue specimens are most frequently done. Micrographic Surgery, Mohs,Micrographic Surgery, Moh's,Micrographic Surgery, Mohs',Moh's Micrographic Surgery,Moh's Surgery,Mohs Micrographic Surgery,Surgery, Moh's Micrographic,Surgery, Mohs,Surgery, Mohs Micrographic,Surgery, Mohs',Micrographic Surgery, Moh,Moh Micrographic Surgery,Moh Surgery,Mohs' Micrographic Surgery,Mohs' Surgery,Surgery, Moh,Surgery, Moh Micrographic,Surgery, Moh's,Surgery, Mohs' Micrographic

Related Publications

J W Franks
December 1993, The Journal of dermatologic surgery and oncology,
J W Franks
October 1989, Dermatologic clinics,
J W Franks
April 1999, Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.],
J W Franks
May 1989, The Journal of dermatologic surgery and oncology,
J W Franks
March 1991, The Journal of dermatologic surgery and oncology,
J W Franks
October 2003, Journal of the American Academy of Dermatology,
J W Franks
January 1992, The British journal of dermatology,
J W Franks
June 2012, Clinical privilege white paper,
J W Franks
January 1993, Clinics in plastic surgery,
J W Franks
June 2009, Journal of visual communication in medicine,
Copied contents to your clipboard!