Biomaterial Database

Determination of serum retinol (vitamin A) by high-speed liquid chromatography. 1976

M G De Ruyter, and A P De Leenheer

We report a fast and simple high-speed liquid-chromatographic assay for serum retinol. Only 100 mul of serum is required. The lower detection limit is 50 mug/liter; linearity was demonstrated up to 1.50 mg/liter. On analyzing a serum pool eight times, a CV of 2.5% was obtained. Values by this method are compared with results obtained by a flurometric method [Clin. Chem. 16, 766(1970)].

UI	MeSH Term	Description	Entries
D008832	Microchemistry	The development and use of techniques and equipment to study or perform chemical reactions, with small quantities of materials, frequently less than a milligram or a milliliter.
D002851	Chromatography, High Pressure Liquid	Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.	Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D013050	Spectrometry, Fluorescence	Measurement of the intensity and quality of fluorescence.	Fluorescence Spectrophotometry,Fluorescence Spectroscopy,Spectrofluorometry,Fluorescence Spectrometry,Spectrophotometry, Fluorescence,Spectroscopy, Fluorescence
D013997	Time Factors	Elements of limited time intervals, contributing to particular results or situations.	Time Series,Factor, Time,Time Factor
D014801	Vitamin A	Retinol and derivatives of retinol that play an essential role in metabolic functioning of the retina, the growth of and differentiation of epithelial tissue, the growth of bone, reproduction, and the immune response. Dietary vitamin A is derived from a variety of CAROTENOIDS found in plants. It is enriched in the liver, egg yolks, and the fat component of dairy products.	Retinol,11-cis-Retinol,3,7-dimethyl-9-(2,6,6-trimethyl-1-cyclohexen-1-yl)-2,4,6,8-nonatetraen-1-ol, (all-E)-Isomer,All-Trans-Retinol,Aquasol A,Vitamin A1,All Trans Retinol

Related Publications

M G De Ruyter, and A P De Leenheer

Rapid determination of retinol (vitamin A) in serum by high pressure liquid chromatography (HPLC).

January 1983, International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition,

M G De Ruyter, and A P De Leenheer

Determination of serum retinol by reversed-phase high-performance liquid chromatography.

April 1995, Journal of chromatography. B, Biomedical applications,

M G De Ruyter, and A P De Leenheer

[Determination of vitamin A in pharmaceutical preparations by high-speed liquid chromatography].

May 1976, Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan,

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Simultaneous determination of eight vitamin D2 isomers by high-speed liquid chromatography.

January 1976, Journal of nutritional science and vitaminology,

M G De Ruyter, and A P De Leenheer

[Determination of bromvalerylurea in serum by high speed liquid chromatography (author's transl)].

June 1980, Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan,