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We describe a gas chromatographic method for measuring submicrogram quantities of dianhydrogalactitol, a promising antineoplastic agent (currently undergoing clinical trials in humans), in plasma. The drug is first extracted from blood plasma by saturating the aqueous phase with solid potassium carbonate and extracting with isopropanol/chloroform (9/1 by vol). It is then converted to the corresponding n-butaneboronic ester by reaction at room temperature with butaneboronic acid and chromatographed on an SE-30 (3%) column, with flame ionization detection. Practicality of the method for monitoring drug distribution was demonstrated by administering dianhydrogalactitol in therapeutic doses to a dog and monitoring its concentrations in blood for the next 2 h.
T Kimura, and
L A Sternson, and
T Higuchi
May 1978,
Cancer treatment reports,
T Kimura, and
L A Sternson, and
T Higuchi
September 1978,
Clinical chemistry,
T Kimura, and
L A Sternson, and
T Higuchi
January 1989,
Cancer chemotherapy and pharmacology,
T Kimura, and
L A Sternson, and
T Higuchi
October 1972,
Cancer chemotherapy reports,
T Kimura, and
L A Sternson, and
T Higuchi
March 1980,
The Biochemical journal,
T Kimura, and
L A Sternson, and
T Higuchi
February 1987,
Yao xue xue bao = Acta pharmaceutica Sinica,
T Kimura, and
L A Sternson, and
T Higuchi
September 1983,
Yao xue xue bao = Acta pharmaceutica Sinica,
T Kimura, and
L A Sternson, and
T Higuchi
January 1976,
Journal of the National Cancer Institute,
T Kimura, and
L A Sternson, and
T Higuchi
January 1972,
Journal of agricultural and food chemistry,
T Kimura, and
L A Sternson, and
T Higuchi
July 1977,
Journal of chromatography,
