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We report an iodine-125 radioimmunoassay for cortisol in biological fluids, in which interfering binding proteins are enzymatically denatured. An antiserum to cortisol-3-carboxymethyloxime-bovine serum albumin, extremely low cross-reacting with other corticosteroids, was raised in rabbits. A cortisol-3-carboxymethyloxime tyrosine methyl ester derivative was synthesized and labeled with iodine-125 by standard radioiodination techniques. To eliminate the need for extraction and recovery procedures, we digested interfering binding witha proteolytic enzyme, which then was heat-inactivated before adding the labeled derivative and the premixed, preincubated antiserum complex. There was quantitative analytical recovery of esogenous cortisol added to sera from a normal man, a normal woman, and a pregnant woman. Values for the same samples agreed after extraction and chromatographic purification and agreed well with values obtained by other techniques by independent reference laboratories. The five-step assay can be done in 6 h or less.
M J Hasler, and
K Painter, and
G D Niswender
November 1976,
Clinical chemistry,
M J Hasler, and
K Painter, and
G D Niswender
July 1975,
Horumon to rinsho. Clinical endocrinology,
M J Hasler, and
K Painter, and
G D Niswender
August 1976,
Kaku igaku. The Japanese journal of nuclear medicine,
M J Hasler, and
K Painter, and
G D Niswender
July 1980,
Clinical chemistry,
M J Hasler, and
K Painter, and
G D Niswender
September 1978,
Clinical chemistry,
M J Hasler, and
K Painter, and
G D Niswender
October 1978,
Pathology,
M J Hasler, and
K Painter, and
G D Niswender
September 1983,
Annals of clinical biochemistry,
M J Hasler, and
K Painter, and
G D Niswender
November 1977,
Horumon to rinsho. Clinical endocrinology,
M J Hasler, and
K Painter, and
G D Niswender
February 1976,
Clinical chemistry,
M J Hasler, and
K Painter, and
G D Niswender
September 1977,
Horumon to rinsho. Clinical endocrinology,
