Biomaterial Database

Characterization of thiamine pyrophosphatase positive phagocytic cells in the neural lobe of rat pituitary. 1998

B Gajkowska, and M Walski, and H Olszewska

Laboratory of the Ultrastructure of the Nervous System, Medical Research Centre, Polish Academy of Sciences, Warszawa.

Here, using a histochemical staining for a microglia/phagocyte marker TPP-ase (Murabe, Sano 1981), and an electron microscopy we characterized the population of pituitary phagocytic cells activated by cerebral ischemia. An intense thiamine pyrophosphatase (TPP-ase) activity was demonstrated in glial cells and some cells of blood vessels of neural lobe, late period (12 months) after experimental ischemia. TPP-ase positive cells were ultrastructurally identified as pituicytes, microglia, pericytes and perivascular cells. The product characteristic for TPP-ase activity was seen on plasma membrane of these cells. Our electron-microscopic histochemical results provide strong support for a role of pituicytes, pericytes and perivascular cells as a phagocytic cells involved in mechanism of elimination of ischemically damaged axonal endings in neural lobe.

UI	MeSH Term	Description	Entries
D010586	Phagocytes	Cells that can carry out the process of PHAGOCYTOSIS.	Phagocyte,Phagocytic Cell,Phagocytic Cells,Cell, Phagocytic,Cells, Phagocytic
D010902	Pituitary Gland	A small, unpaired gland situated in the SELLA TURCICA. It is connected to the HYPOTHALAMUS by a short stalk which is called the INFUNDIBULUM.	Hypophysis,Hypothalamus, Infundibular,Infundibular Stalk,Infundibular Stem,Infundibulum (Hypophysis),Infundibulum, Hypophyseal,Pituitary Stalk,Hypophyseal Infundibulum,Hypophyseal Stalk,Hypophysis Cerebri,Infundibulum,Cerebri, Hypophysis,Cerebrus, Hypophysis,Gland, Pituitary,Glands, Pituitary,Hypophyseal Stalks,Hypophyses,Hypophysis Cerebrus,Infundibular Hypothalamus,Infundibular Stalks,Infundibulums,Pituitary Glands,Pituitary Stalks,Stalk, Hypophyseal,Stalk, Infundibular,Stalks, Hypophyseal,Stalks, Infundibular
D002462	Cell Membrane	The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.	Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D002545	Brain Ischemia	Localized reduction of blood flow to brain tissue due to arterial obstruction or systemic hypoperfusion. This frequently occurs in conjunction with brain hypoxia (HYPOXIA, BRAIN). Prolonged ischemia is associated with BRAIN INFARCTION.	Cerebral Ischemia,Ischemic Encephalopathy,Encephalopathy, Ischemic,Ischemia, Cerebral,Brain Ischemias,Cerebral Ischemias,Ischemia, Brain,Ischemias, Cerebral,Ischemic Encephalopathies
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013834	Thiamine Pyrophosphatase	An enzyme that hydrolyzes thiamine pyrophosphate to thiamine monophosphate plus inorganic phosphate. EC 3.6.1.-.	TPPase,Thiamine Pyrophosphate Phosphohydrolase,Phosphohydrolase, Thiamine Pyrophosphate,Pyrophosphatase, Thiamine,Pyrophosphate Phosphohydrolase, Thiamine
D017208	Rats, Wistar	A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.	Wistar Rat,Rat, Wistar,Wistar Rats
D051381	Rats	The common name for the genus Rattus.	Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus

Related Publications

B Gajkowska, and M Walski, and H Olszewska

Neural lobe of pituitary modulates corticotropin release in the rat.

March 1980, Endocrinology,

B Gajkowska, and M Walski, and H Olszewska

Thiamine pyrophosphatase -- TPPase -- as a "marker enzyme" of the germ cells in the rat.

December 1979, Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme,

B Gajkowska, and M Walski, and H Olszewska

Thiamine pyrophosphatase and nucleoside diphosphatase in rat brain.

January 1984, Biochemical and biophysical research communications,

B Gajkowska, and M Walski, and H Olszewska

Incidence of thiamine pyrophosphatase activity in the segmenting rat ovum.

January 1979, Folia morphologica,

B Gajkowska, and M Walski, and H Olszewska

Subcellular distribution of thiamine pyrophosphatase in rat cerebral cortex.

September 1970, Biochimica et biophysica acta,

B Gajkowska, and M Walski, and H Olszewska

Subcellular distribution of thiamine-pyrophosphokinase and thiamine-pyrophosphatase activities in rat isolated enterocytes.

January 1977, International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition,

B Gajkowska, and M Walski, and H Olszewska

Thiamine pyrophosphatase cytochemistry in rat endometrium during the oestrous cycle.

August 1994, Histochemistry,

B Gajkowska, and M Walski, and H Olszewska

Proliferation of glial cells in vivo induced in the neural lobe of the rat pituitary by lithium.

August 2000, Cell proliferation,

B Gajkowska, and M Walski, and H Olszewska

Ultrastructural changes in the neural lobe of the rat pituitary following nicotine pretreatment.

November 1983, Experimental and clinical endocrinology,

B Gajkowska, and M Walski, and H Olszewska

The partial purification and characterization of thiamine pyrophosphatase from rabbit brain.

July 1972, Journal of neurochemistry,