Biomaterial Database

Identification of CNS neurons innervating the rat prostate: a transneuronal tracing study using pseudorabies virus. 1998

R Orr, and L Marson

Department of Surgery, University of North Carolina School of Medicine, Chapel Hill 27599-7235, USA.

The spinal and brain neurons that innervate the rat prostate were identified using the transneuronal tracing technique. Three groups of rats were prepared: (1) nerve intact, (2) bilateral pelvic nerve cut and right hypogastric nerve cut and (3) bilateral hypogastric nerve cut and right pelvic nerve cut. Pseudorabies virus (PRV) was injected into the ventral prostate on the left side. After 2-4 days, the rats were perfused transcardially under deep anesthesia and the spinal cord and brain removed. PRV-labelled cells were identified using immunohistochemistry. After 3 days survival, sympathetic and parasympathetic preganglionic neurons were labelled with PRV. In addition, spinal interneurons were found in the dorsal gray commissure (DGC) of T13-S1. Rats with only one hypogastric nerve intact resulted in spinal labelling of sympathetic preganglionic neurons in the DGC and ipsilateral intermediolateral cell column (IML). In addition, many spinal interneurons were found from L1 to L6 in the medial gray. Rats with only one pelvic nerve intact displayed PRV-labelled cells in the parasympathetic preganglionic nucleus ipsilateral to the injection site. Spinal interneurons were present in the region of the IML and in the medial cord. In the brain, areas predominately labelled with PRV included the nucleus gigantocellularis and paragigantocellularis, raphe magnus, raphe pallidus, A5, Barrington's nucleus, central gray, ventral tegmental area, the paraventricular nucleus of the hypothalamus, lateral hypothalamus and medial preoptic area. These data demonstrate the sympathetic and parasympathetic spinal circuits and demonstrate the overlap of supraspinal innervation of the spinal interneurons.

UI	MeSH Term	Description	Entries
D007150	Immunohistochemistry	Histochemical localization of immunoreactive substances using labeled antibodies as reagents.	Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D008297	Male		Males
D008722	Methods	A series of steps taken in order to conduct research.	Techniques,Methodological Studies,Methodological Study,Procedures,Studies, Methodological,Study, Methodological,Method,Procedure,Technique
D009474	Neurons	The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.	Nerve Cells,Cell, Nerve,Cells, Nerve,Nerve Cell,Neuron
D010275	Parasympathetic Nervous System	The craniosacral division of the autonomic nervous system. The cell bodies of the parasympathetic preganglionic fibers are in brain stem nuclei and in the sacral spinal cord. They synapse in cranial autonomic ganglia or in terminal ganglia near target organs. The parasympathetic nervous system generally acts to conserve resources and restore homeostasis, often with effects reciprocal to the sympathetic nervous system.	Nervous System, Parasympathetic,Nervous Systems, Parasympathetic,Parasympathetic Nervous Systems,System, Parasympathetic Nervous,Systems, Parasympathetic Nervous
D011467	Prostate	A gland in males that surrounds the neck of the URINARY BLADDER and the URETHRA. It secretes a substance that liquefies coagulated semen. It is situated in the pelvic cavity behind the lower part of the PUBIC SYMPHYSIS, above the deep layer of the triangular ligament, and rests upon the RECTUM.	Prostates
D011558	Herpesvirus 1, Suid	A species of VARICELLOVIRUS producing a respiratory infection (PSEUDORABIES) in swine, its natural host. It also produces an usually fatal ENCEPHALOMYELITIS in cattle, sheep, dogs, cats, foxes, and mink.	Aujeszky's Disease Virus,Swine Herpesvirus 1,Aujeszky Disease Virus,Herpesvirus 1 (alpha), Suid,Herpesvirus Suis,Pseudorabies Virus,Suid Herpesvirus 1,Aujeszkys Disease Virus,Herpesvirus 1, Swine,Pseudorabies Viruses,Virus, Pseudorabies,Viruses, Pseudorabies
D001931	Brain Mapping	Imaging techniques used to colocalize sites of brain functions or physiological activity with brain structures.	Brain Electrical Activity Mapping,Functional Cerebral Localization,Topographic Brain Mapping,Brain Mapping, Topographic,Functional Cerebral Localizations,Mapping, Brain,Mapping, Topographic Brain
D001933	Brain Stem	The part of the brain that connects the CEREBRAL HEMISPHERES with the SPINAL CORD. It consists of the MESENCEPHALON; PONS; and MEDULLA OBLONGATA.	Brainstem,Truncus Cerebri,Brain Stems,Brainstems,Cerebri, Truncus,Cerebrus, Truncus,Truncus Cerebrus
D002490	Central Nervous System	The main information-processing organs of the nervous system, consisting of the brain, spinal cord, and meninges.	Cerebrospinal Axis,Axi, Cerebrospinal,Axis, Cerebrospinal,Central Nervous Systems,Cerebrospinal Axi,Nervous System, Central,Nervous Systems, Central,Systems, Central Nervous