-transparent.png){kind=logo}
The size of the polyadenylate segment of globin messenger RNA isolated from spleens of anaemic rabbits was estimated by comparison of its electrophoretic migration in polyacrylamide gels to that of synthetic poly(A) segments of known lengths. Conditions of enzymic degradation of mRNA with pancreatic ribonuclease and T1 ribonuclease were carefully established in order to ensure complete degradation of the heteropolymeric part of mRNA without affecting the polyadenylate sequence. The poly (A) segments of spleen globin mRNA were found to be 25-90 nucleotides long whilst those of peripheral blood reticulocytes from the same animals were only 10-30 residues long. Since spleen contains young erythroid cells and since anucleated blood reticulocytes constitute a statistically older population of the same cell line, these results support the idea that the poly(A) segment of mRNA shortens when the message ages.
P Nokin, and
A Burny, and
G Huez, and
G Marbaix
February 1974,
Archives internationales de physiologie et de biochimie,
P Nokin, and
A Burny, and
G Huez, and
G Marbaix
June 1974,
Journal of molecular biology,
P Nokin, and
A Burny, and
G Huez, and
G Marbaix
August 1975,
Proceedings of the National Academy of Sciences of the United States of America,
P Nokin, and
A Burny, and
G Huez, and
G Marbaix
April 1976,
Archives internationales de physiologie et de biochimie,
P Nokin, and
A Burny, and
G Huez, and
G Marbaix
November 1975,
European journal of biochemistry,
P Nokin, and
A Burny, and
G Huez, and
G Marbaix
August 1974,
Proceedings of the National Academy of Sciences of the United States of America,
P Nokin, and
A Burny, and
G Huez, and
G Marbaix
June 1973,
Molecular biology reports,
P Nokin, and
A Burny, and
G Huez, and
G Marbaix
May 1977,
The Journal of biological chemistry,
P Nokin, and
A Burny, and
G Huez, and
G Marbaix
January 1976,
Archives internationales de physiologie et de biochimie,
P Nokin, and
A Burny, and
G Huez, and
G Marbaix
May 1978,
European journal of biochemistry,
