Biomaterial Database

Immunohistochemical characterization of a polyclonal antibody against Sphaerospora dicentrarchi (Myxosporea: Bivalvulida), a parasite from sea bass (Dicentrarchus labrax L.) (Teleostei: Serranidae). 1998

P Muñoz, and A Sitjà-Bobadilla, and P Alvarez-Pellitero

Instituto de Acuicultura Torre de la Sal, CSIC, Ribera de Cabanes, Castellón, Spain.

Rabbit antibody was raised against Sphaerospora dicentrarchi, a histozoic parasite of sea bass (Dicentrarchus labrax L.). Light and electron immunohistological staining were used to characterize its specificity and possible reactivity toward other fish parasites. In light immunohistochemistry the polar capsules and valves of the S. dicentrarchi spores appeared strongly stained, whereas developmental stages were not. Electron microscopic histochemistry revealed intense labeling in valves and some developmental stages. Cross-reaction was observed with all the myxosporean parasites assayed, even with those belonging to other genera. Polar capsules of all the myxosporean species except Polysporoplasma sparis were the main structures stained by the polyclonal antibody. These observations could reveal the existence of conserved antigenic epitopes in polar capsules of different Myxosporea.

UI	MeSH Term	Description	Entries
D007150	Immunohistochemistry	Histochemical localization of immunoreactive substances using labeled antibodies as reagents.	Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D008854	Microscopy, Electron	Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.	Electron Microscopy
D010273	Parasitic Diseases, Animal	Animal diseases caused by PARASITES.	Parasitic Infections, Animal,Animal Parasitic Disease,Animal Parasitic Diseases,Animal Parasitic Infection,Animal Parasitic Infections,Disease, Animal Parasitic,Diseases, Animal Parasitic,Infection, Animal Parasitic,Infections, Animal Parasitic,Parasitic Disease, Animal,Parasitic Infection, Animal
D011817	Rabbits	A burrowing plant-eating mammal with hind limbs that are longer than its fore limbs. It belongs to the family Leporidae of the order Lagomorpha, and in contrast to hares, possesses 22 instead of 24 pairs of chromosomes.	Belgian Hare,New Zealand Rabbit,New Zealand Rabbits,New Zealand White Rabbit,Rabbit,Rabbit, Domestic,Chinchilla Rabbits,NZW Rabbits,New Zealand White Rabbits,Oryctolagus cuniculus,Chinchilla Rabbit,Domestic Rabbit,Domestic Rabbits,Hare, Belgian,NZW Rabbit,Rabbit, Chinchilla,Rabbit, NZW,Rabbit, New Zealand,Rabbits, Chinchilla,Rabbits, Domestic,Rabbits, NZW,Rabbits, New Zealand,Zealand Rabbit, New,Zealand Rabbits, New,cuniculus, Oryctolagus
D005393	Fish Diseases	Diseases of freshwater, marine, hatchery or aquarium fish. This term includes diseases of both teleosts (true fish) and elasmobranchs (sharks, rays and skates).	Disease, Fish,Diseases, Fish,Fish Disease
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000913	Antibodies, Protozoan	Immunoglobulins produced in a response to PROTOZOAN ANTIGENS.	Protozoan Antibodies
D001492	Bass	Common name for FISHES belonging to the order Perciformes and occurring in three different families.	Dicentrarchus,Micropterus,Morone,Moronidae,Serranidae,Morone americana,Sea Bass,Sea Basses,Temperate Basses,White Perch,Bass, Sea,Basses, Sea,Basses, Temperate,Perch, White
D013170	Spores	The reproductive elements of lower organisms, such as BACTERIA; FUNGI; and cryptogamic plants.	Spore
D056890	Eukaryota	One of the three domains of life (the others being BACTERIA and ARCHAEA), also called Eukarya. These are organisms whose cells are enclosed in membranes and possess a nucleus. They comprise almost all multicellular and many unicellular organisms, and are traditionally divided into groups (sometimes called kingdoms) including ANIMALS; PLANTS; FUNGI; and various algae and other taxa that were previously part of the old kingdom Protista.	Eukaryotes,Eucarya,Eukarya,Eukaryotas,Eukaryote