BIOMAT Database Logo BIOMAT Database Logo

Photoaffinity labeling of DNA polymerase from Thermus thermophilus and DNA template by photoreactive analogs of dCTP. 1998

A L Zakharenko, and S N Khodyreva, and N I Rechkunova, and I V Safronov, and D V Pyshnyi, and S K Degtyarev, and O I Lavrik
Novosibirsk Institute of Bioorganic Chemistry, Siberian Branch of Russian Academy of Sciences, Novosibirsk, 630090, Russia. lavrik@niboch.nsc.ru

Substrate properties of dCTP analogs N4-[2-(4-azidotetrafluorobenzoylamino)-ethyl]-2;-deoxycytidine-5; -triphosphate (FABdCTP), 5-[N-(4-azidotetrafluorobenzoyl)-3-amino-trans-propen-1-yl] -2;-deoxycytidine-5;-triphosphate (AlFABdCTP), and N4-[2-(2-nitro-5-azidobenzoylamino)-ethyl]-2;-deoxycytidine-5; -triphosphate (NABdCTP) were studied in the reaction of DNA synthesis catalyzed by DNA polymerase from the extremely thermophilic bacterium Thermus thermophilus B 35 (Tte DNA polymerase). The enzyme was photoaffinity labeled with the mentioned derivatives, NABdCTP being used for the first time. The photoreactive primers containing FABdCTP and AlFABdCTP were synthesized in situ by Tte DNA polymerase and used in the complementary addressed labeling of DNA template. The efficiency of DNA template labeling is shown to be a function of the structure of the photoactive group.

UI MeSH Term Description Entries
D010758 Phosphorus A non-metal element that has the atomic symbol P, atomic number 15, and atomic weight 31. It is an essential element that takes part in a broad variety of biochemical reactions. Black Phosphorus,Phosphorus-31,Red Phosphorus,White Phosphorus,Yellow Phosphorus,Phosphorus 31,Phosphorus, Black,Phosphorus, Red,Phosphorus, White,Phosphorus, Yellow
D010782 Photolysis Chemical bond cleavage reactions resulting from absorption of radiant energy. Photodegradation
D002384 Catalysis The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction. Catalyses
D003845 Deoxycytosine Nucleotides Cytosine nucleotides which contain deoxyribose as the sugar moiety. Deoxycytidine Phosphates,Nucleotides, Deoxycytosine,Phosphates, Deoxycytidine
D004259 DNA-Directed DNA Polymerase DNA-dependent DNA polymerases found in bacteria, animal and plant cells. During the replication process, these enzymes catalyze the addition of deoxyribonucleotide residues to the end of a DNA strand in the presence of DNA as template-primer. They also possess exonuclease activity and therefore function in DNA repair. DNA Polymerase,DNA Polymerases,DNA-Dependent DNA Polymerases,DNA Polymerase N3,DNA Dependent DNA Polymerases,DNA Directed DNA Polymerase,DNA Polymerase, DNA-Directed,DNA Polymerases, DNA-Dependent,Polymerase N3, DNA,Polymerase, DNA,Polymerase, DNA-Directed DNA,Polymerases, DNA,Polymerases, DNA-Dependent DNA
D000345 Affinity Labels Analogs of those substrates or compounds which bind naturally at the active sites of proteins, enzymes, antibodies, steroids, or physiological receptors. These analogs form a stable covalent bond at the binding site, thereby acting as inhibitors of the proteins or steroids. Affinity Labeling Reagents,Labeling Reagents, Affinity,Labels, Affinity,Reagents, Affinity Labeling
D001386 Azides Organic or inorganic compounds that contain the -N3 group. Azide
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D013698 Templates, Genetic Macromolecular molds for the synthesis of complementary macromolecules, as in DNA REPLICATION; GENETIC TRANSCRIPTION of DNA to RNA, and GENETIC TRANSLATION of RNA into POLYPEPTIDES. Genetic Template,Genetic Templates,Template, Genetic
D016963 Thermus thermophilus A species of gram-negative, aerobic, rod-shaped bacteria found in hot springs of neutral to alkaline pH, as well as in hot-water heaters.

Related Publications

A L Zakharenko, and S N Khodyreva, and N I Rechkunova, and I V Safronov, and D V Pyshnyi, and S K Degtyarev, and O I Lavrik
Highly selective affinity labeling of DNA-polymerase from Thermus thermophilus B35 by a binary system of photoreactive agents.
February 2000, Biochemistry. Biokhimiia,
A L Zakharenko, and S N Khodyreva, and N I Rechkunova, and I V Safronov, and D V Pyshnyi, and S K Degtyarev, and O I Lavrik
Affinity modification of human immunodeficiency virus reverse transcriptase and DNA template by photoreactive dCTP analogs.
November 1994, FEBS letters,
A L Zakharenko, and S N Khodyreva, and N I Rechkunova, and I V Safronov, and D V Pyshnyi, and S K Degtyarev, and O I Lavrik
[Photoreactive dTTP analogues as substrates for thermostable DNA polymerase from Thermus thermophilus B35].
January 2004, Bioorganicheskaia khimiia,
A L Zakharenko, and S N Khodyreva, and N I Rechkunova, and I V Safronov, and D V Pyshnyi, and S K Degtyarev, and O I Lavrik
[The use of DNA polymerase from Thermus thermophilus].
January 1992, Voprosy virusologii,
A L Zakharenko, and S N Khodyreva, and N I Rechkunova, and I V Safronov, and D V Pyshnyi, and S K Degtyarev, and O I Lavrik
DNA sequencing with thermostable Tet DNA polymerase from Thermus thermophilus.
December 1989, Nucleic acids research,
A L Zakharenko, and S N Khodyreva, and N I Rechkunova, and I V Safronov, and D V Pyshnyi, and S K Degtyarev, and O I Lavrik
Elongation of repetitive DNA by DNA polymerase from a hyperthermophilic bacterium Thermus thermophilus.
October 2000, Nucleic acids research,
A L Zakharenko, and S N Khodyreva, and N I Rechkunova, and I V Safronov, and D V Pyshnyi, and S K Degtyarev, and O I Lavrik
Reverse transcription and DNA amplification by a Thermus thermophilus DNA polymerase.
August 1991, Biochemistry,
A L Zakharenko, and S N Khodyreva, and N I Rechkunova, and I V Safronov, and D V Pyshnyi, and S K Degtyarev, and O I Lavrik
Long-Range PCR Amplification of DNA by DNA Polymerase III Holoenzyme from Thermus thermophilus.
January 2015, Enzyme research,
A L Zakharenko, and S N Khodyreva, and N I Rechkunova, and I V Safronov, and D V Pyshnyi, and S K Degtyarev, and O I Lavrik
Crystallisation of DNA-dependent RNA polymerase from Thermus thermophilus HB 8.
June 1976, Nature,
A L Zakharenko, and S N Khodyreva, and N I Rechkunova, and I V Safronov, and D V Pyshnyi, and S K Degtyarev, and O I Lavrik
RNA template-dependent 5' nuclease activity of Thermus aquaticus and Thermus thermophilus DNA polymerases.
August 2000, The Journal of biological chemistry,
Copied contents to your clipboard!