Biomaterial Database

The NH2-terminal region of the beta chain of sickle hemoglobin. I. Synthesis and purification of oligopeptides. 1976

A Eastlake, and J G Curd, and A N Schechter

Five peptides from the NH2-terminal region of the beta chain of hemoglobin S, betaS (1-13), betaS (3-13), betaS (1-8), betaS (4-10), And betaS (4-8), have been synthesized by a rapid solid phase method based on the Merrifield procedure. In addition, one peptide, betaS (3-13), has also been synthesized by the original Merrifield method. We have shown that the products of the two methods are comparable, that gel filtration is a useful method for removing truncated fragments of the desired oligopeptide, and that measurement of the efficiency of coupling at each step is an important adjunct to amino acid analysis in determining purity. Peptides of the purity achieved by these methods may be used to fractionate antibodies to the native hemoglobin S, in the characterization of antigen-binding properties of specific antibodies, and in other studies of peptide-protein interactions.

UI	MeSH Term	Description	Entries
D009842	Oligopeptides	Peptides composed of between two and twelve amino acids.	Oligopeptide
D010446	Peptide Fragments	Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.	Peptide Fragment,Fragment, Peptide,Fragments, Peptide
D006451	Hemoglobin, Sickle	An abnormal hemoglobin resulting from the substitution of valine for glutamic acid at position 6 of the beta chain of the globin moiety. The heterozygous state results in sickle cell trait, the homozygous in sickle cell anemia.	Hemoglobin S,Deoxygenated Sickle Hemoglobin,Deoxyhemoglobin S,Hemoglobin SS,Hemoglobin, Deoxygenated Sickle,SS, Hemoglobin,Sickle Hemoglobin,Sickle Hemoglobin, Deoxygenated
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000595	Amino Acid Sequence	The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.	Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000596	Amino Acids	Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.	Amino Acid,Acid, Amino,Acids, Amino