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Previous experimental observations indicate that inhibition of voltage-dependent K+ currents suppresses proliferation of normal Schwann cells. In the present study we tested the opposite relationship, i.e., whether Schwann cells from tumors with abnormally high rates of proliferation would have an increase in membrane K+ currents. Whole-cell membrane currents were studied in cultured cells from schwannomas of two neurofibromatosis type 2 (NF2) patients (n = 53), one patient with a sporadic schwannoma (n = 22), and two control subjects (n = 41). Five different types of voltage-dependent membrane currents were found in all of the Schwann cells tested. Membrane depolarization activated outward K+ and Cl- currents; quinidine was found to block the K+ current (IC50 approximately 1 microM), and NPPB reduced the Cl- current. Ba2+-sensitive inward rectifier K+ currents, fast Na+ currents, and a transient, inactivating K+ current were less frequently observed. On average, NF2 cells were found to have statistically significant higher membrane potential and larger non-inactivating K+ outward current as compared to controls. Electrophysiological parameters of Schwann cells from a sporadic schwannoma showed a tendency for larger outward currents; however, the difference did not reach statistical significance. Together the data support the suggestion of a possible link between K+ outward current and proliferation of Schwann cells.
M Kamleiter, and
C O Hanemann, and
L Kluwe, and
C Rosenbaum, and
S Wosch, and
V F Mautner, and
H W Müller, and
P Grafe
November 2003,
Journal of experimental zoology. Part A, Comparative experimental biology,
M Kamleiter, and
C O Hanemann, and
L Kluwe, and
C Rosenbaum, and
S Wosch, and
V F Mautner, and
H W Müller, and
P Grafe
March 1993,
Biochemical and biophysical research communications,
M Kamleiter, and
C O Hanemann, and
L Kluwe, and
C Rosenbaum, and
S Wosch, and
V F Mautner, and
H W Müller, and
P Grafe
October 1989,
Brain research,
M Kamleiter, and
C O Hanemann, and
L Kluwe, and
C Rosenbaum, and
S Wosch, and
V F Mautner, and
H W Müller, and
P Grafe
March 1995,
Nagoya journal of medical science,
M Kamleiter, and
C O Hanemann, and
L Kluwe, and
C Rosenbaum, and
S Wosch, and
V F Mautner, and
H W Müller, and
P Grafe
May 1999,
Nagoya journal of medical science,
M Kamleiter, and
C O Hanemann, and
L Kluwe, and
C Rosenbaum, and
S Wosch, and
V F Mautner, and
H W Müller, and
P Grafe
April 1977,
Science (New York, N.Y.),
M Kamleiter, and
C O Hanemann, and
L Kluwe, and
C Rosenbaum, and
S Wosch, and
V F Mautner, and
H W Müller, and
P Grafe
October 1990,
Bioscience reports,
M Kamleiter, and
C O Hanemann, and
L Kluwe, and
C Rosenbaum, and
S Wosch, and
V F Mautner, and
H W Müller, and
P Grafe
February 1985,
Proceedings of the National Academy of Sciences of the United States of America,
M Kamleiter, and
C O Hanemann, and
L Kluwe, and
C Rosenbaum, and
S Wosch, and
V F Mautner, and
H W Müller, and
P Grafe
January 1985,
Nature,
M Kamleiter, and
C O Hanemann, and
L Kluwe, and
C Rosenbaum, and
S Wosch, and
V F Mautner, and
H W Müller, and
P Grafe
November 2003,
The Journal of membrane biology,
