BIOMAT Database Logo BIOMAT Database Logo

Enhancement of human immunodeficiency virus type 1 infectivity by Nef is producer cell-dependent. 1998

K Tokunaga, and A Kojima, and T Kurata, and K Ikuta, and H Akari, and A H Koyama, and M Kawamura, and R Inubushi, and R Shimano, and A Adachi
Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan.

The growth kinetics of wild-type and nef mutant viruses of human immunodeficiency virus type 1 were comparatively analysed in several human CD4+ cell lines. Delayed replication of nef mutant virus was observed in all cell lines examined. To determine the stage in the virus replication cycle that is affected by Nef, a single-round replication assay was performed. Initially, the expression of marker genes in transfected cells was examined in order to study the role of Nef in the late phase of infection. The results obtained indicated that Nef is dispensable during the transcription to virion production stage. Next, the effect of Nef on the early phase was investigated with a single-round infection. It was demonstrated that Nef is required in the early phase of the virus replication cycle, from virion adsorption to integration. Finally, the infectivity of virus stocks prepared from four cell lines was determined. The relative infectivity of the nef mutant from the four cell lines differed. Taken together, we conclude that Nef acts via modulation of viral particles to enhance virus infectivity in a cell-dependent manner.

UI MeSH Term Description Entries
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D014407 Tumor Cells, Cultured Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely. Cultured Tumor Cells,Neoplastic Cells, Cultured,Cultured Neoplastic Cells,Cell, Cultured Neoplastic,Cell, Cultured Tumor,Cells, Cultured Neoplastic,Cells, Cultured Tumor,Cultured Neoplastic Cell,Cultured Tumor Cell,Neoplastic Cell, Cultured,Tumor Cell, Cultured
D014771 Virion The infective system of a virus, composed of the viral genome, a protein core, and a protein coat called a capsid, which may be naked or enclosed in a lipoprotein envelope called the peplos. Virus Particle,Viral Particle,Viral Particles,Particle, Viral,Particle, Virus,Particles, Viral,Particles, Virus,Virions,Virus Particles
D014779 Virus Replication The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle. Viral Replication,Replication, Viral,Replication, Virus,Replications, Viral,Replications, Virus,Viral Replications,Virus Replications
D015497 HIV-1 The type species of LENTIVIRUS and the etiologic agent of AIDS. It is characterized by its cytopathic effect and affinity for the T4-lymphocyte. Human immunodeficiency virus 1,HIV-I,Human Immunodeficiency Virus Type 1,Immunodeficiency Virus Type 1, Human
D015693 Gene Products, nef Products of the retroviral NEF GENE. They play a role as accessory proteins that influence the rate of viral infectivity and the destruction of the host immune system. nef gene products were originally found as factors that trans-suppress viral replication and function as negative regulators of transcription. nef stands for negative factor. nef Gene Products,nef Protein,3'-orf Protein,Gene Product, nef,3' orf Protein,nef Gene Product
D054311 nef Gene Products, Human Immunodeficiency Virus Proteins encoded by the NEF GENES of the HUMAN IMMUNODEFICIENCY VIRUS. nef Protein, Human Immunodeficiency Virus,HIV-3'-orf Protein,nef Protein, HIV,HIV 3' orf Protein,HIV nef Protein

Related Publications

K Tokunaga, and A Kojima, and T Kurata, and K Ikuta, and H Akari, and A H Koyama, and M Kawamura, and R Inubushi, and R Shimano, and A Adachi
Producer-cell modification of human immunodeficiency virus type 1: Nef is a virion protein.
July 1996, Journal of virology,
K Tokunaga, and A Kojima, and T Kurata, and K Ikuta, and H Akari, and A H Koyama, and M Kawamura, and R Inubushi, and R Shimano, and A Adachi
Mechanistic independence of Nef and cyclophilin A enhancement of human immunodeficiency virus type 1 infectivity.
August 1998, Virology,
K Tokunaga, and A Kojima, and T Kurata, and K Ikuta, and H Akari, and A H Koyama, and M Kawamura, and R Inubushi, and R Shimano, and A Adachi
Infectivity enhancement by human immunodeficiency virus type 1 Nef is independent of its association with a cellular serine/threonine kinase.
December 1997, Journal of virology,
K Tokunaga, and A Kojima, and T Kurata, and K Ikuta, and H Akari, and A H Koyama, and M Kawamura, and R Inubushi, and R Shimano, and A Adachi
Dissociation of the CD4 downregulation and viral infectivity enhancement functions of human immunodeficiency virus type 1 Nef.
July 1995, Journal of virology,
K Tokunaga, and A Kojima, and T Kurata, and K Ikuta, and H Akari, and A H Koyama, and M Kawamura, and R Inubushi, and R Shimano, and A Adachi
Nef enhances human immunodeficiency virus type 1 infectivity in the absence of matrix.
July 2002, Journal of virology,
K Tokunaga, and A Kojima, and T Kurata, and K Ikuta, and H Akari, and A H Koyama, and M Kawamura, and R Inubushi, and R Shimano, and A Adachi
Inefficient enhancement of viral infectivity and CD4 downregulation by human immunodeficiency virus type 1 Nef from Japanese long-term nonprogressors.
June 2002, Journal of virology,
K Tokunaga, and A Kojima, and T Kurata, and K Ikuta, and H Akari, and A H Koyama, and M Kawamura, and R Inubushi, and R Shimano, and A Adachi
Human immunodeficiency virus type 1 Nef incorporation into virions does not increase infectivity.
January 2009, Journal of virology,
K Tokunaga, and A Kojima, and T Kurata, and K Ikuta, and H Akari, and A H Koyama, and M Kawamura, and R Inubushi, and R Shimano, and A Adachi
Nef from human immunodeficiency virus type 1(F12) inhibits viral production and infectivity.
July 2001, Journal of virology,
K Tokunaga, and A Kojima, and T Kurata, and K Ikuta, and H Akari, and A H Koyama, and M Kawamura, and R Inubushi, and R Shimano, and A Adachi
Dendritic cell-mediated trans-enhancement of human immunodeficiency virus type 1 infectivity is independent of DC-SIGN.
March 2007, Journal of virology,
K Tokunaga, and A Kojima, and T Kurata, and K Ikuta, and H Akari, and A H Koyama, and M Kawamura, and R Inubushi, and R Shimano, and A Adachi
Nef can enhance the infectivity of receptor-pseudotyped human immunodeficiency virus type 1 particles.
November 2008, Journal of virology,
Copied contents to your clipboard!