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A rapid enzyme immunoassay membrane test, Directigen Flu A (Becton Dickinson, USA), was evaluated by using virus isolates and clinical specimens. The reference laboratory diagnosis was based on the results of virus isolation. Directigen Flu A was reactive for all subtypes of human influenza A viruses, including reference strains of H1N1, H2N2 and H3N2. Moreover, H5N1 (Hongkong/156/97) was also detected by this kit. No cross reactivity was detected with other respiratory viruses. Directigen Flu A showed positive reaction with the solution containing influenza A virus of 2.4 x 10(3) pfu/assay. The rapid test demonstrated 77.9% sensitivity and 98.4% specificity for testing of throat swabs from children with respiratory symptoms. It showed higher sensitivity and specificity (92.1% and 100%) for testing of nasopharyngeal aspirates. Directigen Flu A should be useful for the rapid diagnosis of influenza A virus infection.
H Shimizu, and
S Watanabe, and
C Kawakami, and
Y Hirai, and
K Kimura, and
N Sugaya, and
M Imai
October 1992,
Journal of virological methods,
H Shimizu, and
S Watanabe, and
C Kawakami, and
Y Hirai, and
K Kimura, and
N Sugaya, and
M Imai
January 1993,
Journal of clinical microbiology,
H Shimizu, and
S Watanabe, and
C Kawakami, and
Y Hirai, and
K Kimura, and
N Sugaya, and
M Imai
February 2015,
Journal of virological methods,
H Shimizu, and
S Watanabe, and
C Kawakami, and
Y Hirai, and
K Kimura, and
N Sugaya, and
M Imai
January 1990,
Research in virology,
H Shimizu, and
S Watanabe, and
C Kawakami, and
Y Hirai, and
K Kimura, and
N Sugaya, and
M Imai
April 1991,
Pathologie-biologie,
H Shimizu, and
S Watanabe, and
C Kawakami, and
Y Hirai, and
K Kimura, and
N Sugaya, and
M Imai
November 1995,
Diagnostic microbiology and infectious disease,
H Shimizu, and
S Watanabe, and
C Kawakami, and
Y Hirai, and
K Kimura, and
N Sugaya, and
M Imai
November 1999,
Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases,
H Shimizu, and
S Watanabe, and
C Kawakami, and
Y Hirai, and
K Kimura, and
N Sugaya, and
M Imai
October 2001,
Enfermedades infecciosas y microbiologia clinica,
H Shimizu, and
S Watanabe, and
C Kawakami, and
Y Hirai, and
K Kimura, and
N Sugaya, and
M Imai
April 1991,
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc,
H Shimizu, and
S Watanabe, and
C Kawakami, and
Y Hirai, and
K Kimura, and
N Sugaya, and
M Imai
June 1997,
Journal of clinical microbiology,
