Aim of the present study was to evaluate the effect of acute hyperglycemia on peripheral nerve conduction measurements. Five healthy male volunteers aged 36-42 years underwent nerve conduction studies during hyperglycemia (blood glucose approximately 12 mmol/l) induced by intravenous infusion of glucose and maintained for 120 minutes. Peroneal motor and sural sensory nerve conduction velocities and amplitudes were measured from the right leg at 15 min intervals starting at 15 min before and continuing 30 min after glucose infusion. Data were analysed using paired t-test comparing measurements at each time point after the start of the infusion to the second control measurement immediately prior to the infusion. All nerve conduction velocities and amplitudes were similar before, during and after induced hyperglycemia. The results suggest that it is unnecessary to standardise blood glucose concentration during measurement of peripheral nerve functions.