Biomaterial Database

A novel method for determining kinetic parameters of dissociating enzyme systems. 1998

Z X Wang

National Laboratory of Biomacromolecules, Institute of Biophysics, Academia Sinica, Beijing, 100101, People's Republic of China.

The theoretical analysis has been presented for the kinetics of dissociating-associating enzyme-catalyzed reactions. On the basis of the kinetic equation of substrate reaction, a general procedure is developed for determining the kinetic constants of dissociating-associating enzyme reactions. By analyzing the experimental data of initial velocity and steady-state velocity as functions of enzyme and substrate concentration, all unknown kinetic parameters can be determined from several simple, sequential calculations. This method is simple and rigorous, and the required experiments may also not be difficult for most dissociating enzyme systems. Therefore, the present method should be a useful addition to the available methods for studying subunit dissociation of enzymes. In comparison to other physical methods, the advantage of this method is not only its usefulness in the study of self-associating reactions at very low protein concentration but its convenience in the study of substrate effects on subunit-subunit interactions.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008024	Ligands	A molecule that binds to another molecule, used especially to refer to a small molecule that binds specifically to a larger molecule, e.g., an antigen binding to an antibody, a hormone or neurotransmitter binding to a receptor, or a substrate or allosteric effector binding to an enzyme. Ligands are also molecules that donate or accept a pair of electrons to form a coordinate covalent bond with the central metal atom of a coordination complex. (From Dorland, 27th ed)	Ligand
D008956	Models, Chemical	Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.	Chemical Models,Chemical Model,Model, Chemical
D011487	Protein Conformation	The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).	Conformation, Protein,Conformations, Protein,Protein Conformations
D004798	Enzymes	Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however CATALYTIC RNA and CATALYTIC DNA molecules have also been identified.	Biocatalyst,Enzyme,Biocatalysts