Quantification of the secretory glycoproteins of the subcommissural organ by a sensitive sandwich ELISA with a polyclonal antibody and a set of monoclonal antibodies against the bovine Reissner's fiber. 1998

G Estivill-Torrús, and M Cifuentes, and J M Grondona, and E Miranda, and F J Bermúdez-Silva, and P Fernández-Llebrez, and J Pérez
Departamento de Biología Animal, Facultad de Ciencias, Universidad de Málaga, E-29071 Málaga, Spain.

The subcommissural organ (SCO) is an ependymal brain gland that releases glycoproteins into the ventricular cerebrospinal fluid where they condense to form the Reissner's fiber (RF). We have developed a highly sensitive and specific two-antibody sandwich enzyme-linked immunosorbent assay (ELISA) for the quantification of the bovine SCO secretory material. The assay was based on the use of the IgG fraction of a polyclonal antiserum against the bovine RF as capture antibody and a pool of three peroxidase-labeled monoclonal antibodies that recognize non-overlapping epitopes of the RF glycoproteins as detection antibody. The detection limit was 1 ng/ml and the working range extended from 1 to 4000 ng/ml. The calibration curve, generated with RF glycoproteins, showed two linear segments: one of low sensitivity, ranging from 1 to 125 ng/ml, and the other of high sensitivity between 125 and 4000 ng/ml. This assay was highly reproducible (mean intra- and interassay coefficient of variation 2.2% and 5.3%, respectively) and its detectability and sensitivity were higher than those of ELISAs using exclusively either polyclonal or monoclonal antibodies against RF glycoproteins. The assay succeeded in detecting and measuring secretory material in crude extracts of bovine SCO, culture medium supernatant of SCO explants and incubation medium of bovine RF; however, soluble secretory material was not detected in bovine cerebrospinal fluid.

UI MeSH Term Description Entries
D009419 Nerve Tissue Proteins Proteins, Nerve Tissue,Tissue Proteins, Nerve
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000906 Antibodies Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D000918 Antibody Specificity The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site. Antibody Specificities,Specificities, Antibody,Specificity, Antibody
D013351 Subcommissural Organ Ependymal derivative located at the junction of the THIRD VENTRICLE and the CEREBRAL AQUEDUCT; and the SOMATOSTATIN SECRETING CELLS. Organ, Subcommissural,Organs, Subcommissural,Subcommissural Organs
D015816 Cell Adhesion Molecules, Neuronal Surface ligands that mediate cell-to-cell adhesion and function in the assembly and interconnection of the vertebrate nervous system. These molecules promote cell adhesion via a homophilic mechanism. These are not to be confused with NEURAL CELL ADHESION MOLECULES, now known to be expressed in a variety of tissues and cell types in addition to nervous tissue. Axon-Associated Adhesion Molecules,Neuronal Cell Adhesion Molecules,Adhesion Molecules, Axon-Associated,Axon Associated Adhesion Molecules,Molecules, Axon-Associated Adhesion
D017077 Culture Media, Conditioned Culture media containing biologically active components obtained from previously cultured cells or tissues that have released into the media substances affecting certain cell functions (e.g., growth, lysis). Conditioned Culture Media,Conditioned Culture Medium,Conditioned Media,Conditioned Medium,Culture Medium, Conditioned,Media, Conditioned,Medium, Conditioned

Related Publications

G Estivill-Torrús, and M Cifuentes, and J M Grondona, and E Miranda, and F J Bermúdez-Silva, and P Fernández-Llebrez, and J Pérez
October 1996, Cell and tissue research,
G Estivill-Torrús, and M Cifuentes, and J M Grondona, and E Miranda, and F J Bermúdez-Silva, and P Fernández-Llebrez, and J Pérez
October 1991, Brain research. Molecular brain research,
G Estivill-Torrús, and M Cifuentes, and J M Grondona, and E Miranda, and F J Bermúdez-Silva, and P Fernández-Llebrez, and J Pérez
January 1988, Cell and tissue research,
G Estivill-Torrús, and M Cifuentes, and J M Grondona, and E Miranda, and F J Bermúdez-Silva, and P Fernández-Llebrez, and J Pérez
October 1999, Journal of immunological methods,
G Estivill-Torrús, and M Cifuentes, and J M Grondona, and E Miranda, and F J Bermúdez-Silva, and P Fernández-Llebrez, and J Pérez
January 1988, Cell and tissue research,
G Estivill-Torrús, and M Cifuentes, and J M Grondona, and E Miranda, and F J Bermúdez-Silva, and P Fernández-Llebrez, and J Pérez
December 2006, Cell and tissue research,
G Estivill-Torrús, and M Cifuentes, and J M Grondona, and E Miranda, and F J Bermúdez-Silva, and P Fernández-Llebrez, and J Pérez
June 2000, Cell and tissue research,
G Estivill-Torrús, and M Cifuentes, and J M Grondona, and E Miranda, and F J Bermúdez-Silva, and P Fernández-Llebrez, and J Pérez
January 2016, Journal of immunoassay & immunochemistry,
G Estivill-Torrús, and M Cifuentes, and J M Grondona, and E Miranda, and F J Bermúdez-Silva, and P Fernández-Llebrez, and J Pérez
September 1990, Brain research. Developmental brain research,
G Estivill-Torrús, and M Cifuentes, and J M Grondona, and E Miranda, and F J Bermúdez-Silva, and P Fernández-Llebrez, and J Pérez
January 1990, Experimental brain research,
Copied contents to your clipboard!