-transparent.png){kind=logo}
Lactoferrin (LF) is an iron-binding glycoprotein present in various secretions including milk and the specific granules of neutrophils. The main biological properties of this protein are thought to concern the regulation of iron absorption, antimicrobial activity and modulation of neutrophil activity. Copper bound LF (Cu-LF) inhibited the stimulation-dependent reduction of cytochrome c (Cyt. c) in guinea pig peritoneal neutrophils (GPMN) but were without effect on NADPH oxidase activity of the respiratory burst. However, Cu-LF stimulated the stimulation-dependent production of hydrogen peroxide as seen with superoxide dismutase (SOD). Similar but weaker inhibition of Cyt. c reduction than that shown by Cu-LF was observed with manganese-LF (Mn-LF) but not with ferrous-LF (Fe-LF) or apo-LF (Apo-LF). The inhibitory activity was concentration-dependent and the ID50s of Cu-LF and of Mn-LF were 0.1 and 5 microM, respectively. Reactive oxygen species (ROS) detected by luminol chemiluminescence (LCL) of stimulated-GPMN were partially inhibited by Cu-LF. Changes in LCL of stimulated GPMN induced by Cu-LF were similar to those of superoxide dismutase (SOD). Thus, it is concluded that low concentrations of Cu-LF had SOD-like activity and high concentrations of Cu-LF inhibited the stimulation-dependent generation of ROS.
R Ishisaka, and
T Kanno, and
H Kanematsu, and
T Utsumi, and
J Akiyama, and
A A Horton, and
T Yoshioka
January 1990,
Farmaco (Societa chimica italiana : 1989),
R Ishisaka, and
T Kanno, and
H Kanematsu, and
T Utsumi, and
J Akiyama, and
A A Horton, and
T Yoshioka
January 1993,
Biological trace element research,
R Ishisaka, and
T Kanno, and
H Kanematsu, and
T Utsumi, and
J Akiyama, and
A A Horton, and
T Yoshioka
December 1981,
The Biochemical journal,
R Ishisaka, and
T Kanno, and
H Kanematsu, and
T Utsumi, and
J Akiyama, and
A A Horton, and
T Yoshioka
October 2009,
Dalton transactions (Cambridge, England : 2003),
R Ishisaka, and
T Kanno, and
H Kanematsu, and
T Utsumi, and
J Akiyama, and
A A Horton, and
T Yoshioka
December 1996,
Redox report : communications in free radical research,
R Ishisaka, and
T Kanno, and
H Kanematsu, and
T Utsumi, and
J Akiyama, and
A A Horton, and
T Yoshioka
December 1984,
Agents and actions,
R Ishisaka, and
T Kanno, and
H Kanematsu, and
T Utsumi, and
J Akiyama, and
A A Horton, and
T Yoshioka
September 1975,
Biochemical and biophysical research communications,
R Ishisaka, and
T Kanno, and
H Kanematsu, and
T Utsumi, and
J Akiyama, and
A A Horton, and
T Yoshioka
December 2006,
Bioorganic & medicinal chemistry letters,
R Ishisaka, and
T Kanno, and
H Kanematsu, and
T Utsumi, and
J Akiyama, and
A A Horton, and
T Yoshioka
May 2005,
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie,
R Ishisaka, and
T Kanno, and
H Kanematsu, and
T Utsumi, and
J Akiyama, and
A A Horton, and
T Yoshioka
January 1992,
Romanian journal of neurology and psychiatry = Revue roumaine de neurologie et psychiatrie,
