BIOMAT Database Logo BIOMAT Database Logo

An antagonist peptide-EPO receptor complex suggests that receptor dimerization is not sufficient for activation. 1998

O Livnah, and D L Johnson, and E A Stura, and F X Farrell, and F P Barbone, and Y You, and K D Liu, and M A Goldsmith, and W He, and C D Krause, and S Pestka, and L K Jolliffe, and I A Wilson
Department of Molecular Biology and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California 92037, USA.

Dimerization of the erythropoietin (EPO) receptor (EPOR), in the presence of either natural (EPO) or synthetic (EPO-mimetic peptides, EMPs) ligands is the principal extracellular event that leads to receptor activation. The crystal structure of the extracellular domain of EPOR bound to an inactive (antagonist) peptide at 2.7 A resolution has unexpectedly revealed that dimerization still occurs, but the orientation between receptor molecules is altered relative to active (agonist) peptide complexes. Comparison of the biological properties of agonist and antagonist EMPs with EPO suggests that the extracellular domain orientation is tightly coupled to the cytoplasmic signaling events and, hence, provides valuable new insights into the design of synthetic ligands for EPOR and other cytokine receptors.

UI MeSH Term Description Entries
D008894 Milk Proteins The major protein constituents of milk are CASEINS and whey proteins such as LACTALBUMIN and LACTOGLOBULINS. IMMUNOGLOBULINS occur in high concentrations in COLOSTRUM and in relatively lower concentrations in milk. (Singleton and Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed, p554) Milk Protein,Protein, Milk,Proteins, Milk
D008958 Models, Molecular Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures. Molecular Models,Model, Molecular,Molecular Model
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D010456 Peptides, Cyclic Peptides whose amino acid residues are linked together forming a circular chain. Some of them are ANTI-INFECTIVE AGENTS; some are biosynthesized non-ribosomally (PEPTIDE BIOSYNTHESIS, NON-RIBOSOMAL). Circular Peptide,Cyclic Peptide,Cyclic Peptides,Cyclopeptide,Orbitide,Circular Peptides,Cyclopeptides,Orbitides,Peptide, Circular,Peptide, Cyclic,Peptides, Circular
D010766 Phosphorylation The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety. Phosphorylations
D011487 Protein Conformation The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain). Conformation, Protein,Conformations, Protein,Protein Conformations
D011993 Recombinant Fusion Proteins Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes. Fusion Proteins, Recombinant,Recombinant Chimeric Protein,Recombinant Fusion Protein,Recombinant Hybrid Protein,Chimeric Proteins, Recombinant,Hybrid Proteins, Recombinant,Recombinant Chimeric Proteins,Recombinant Hybrid Proteins,Chimeric Protein, Recombinant,Fusion Protein, Recombinant,Hybrid Protein, Recombinant,Protein, Recombinant Chimeric,Protein, Recombinant Fusion,Protein, Recombinant Hybrid,Proteins, Recombinant Chimeric,Proteins, Recombinant Fusion,Proteins, Recombinant Hybrid
D004268 DNA-Binding Proteins Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases. DNA Helix Destabilizing Proteins,DNA-Binding Protein,Single-Stranded DNA Binding Proteins,DNA Binding Protein,DNA Single-Stranded Binding Protein,SS DNA BP,Single-Stranded DNA-Binding Protein,Binding Protein, DNA,DNA Binding Proteins,DNA Single Stranded Binding Protein,DNA-Binding Protein, Single-Stranded,Protein, DNA-Binding,Single Stranded DNA Binding Protein,Single Stranded DNA Binding Proteins
D004921 Erythropoietin Glycoprotein hormone, secreted chiefly by the KIDNEY in the adult and the LIVER in the FETUS, that acts on erythroid stem cells of the BONE MARROW to stimulate proliferation and differentiation.
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

Related Publications

O Livnah, and D L Johnson, and E A Stura, and F X Farrell, and F P Barbone, and Y You, and K D Liu, and M A Goldsmith, and W He, and C D Krause, and S Pestka, and L K Jolliffe, and I A Wilson
Dimerization of soluble major histocompatibility complex-peptide complexes is sufficient for activation of T cell hybridoma and induction of unresponsiveness.
August 1995, The Journal of experimental medicine,
O Livnah, and D L Johnson, and E A Stura, and F X Farrell, and F P Barbone, and Y You, and K D Liu, and M A Goldsmith, and W He, and C D Krause, and S Pestka, and L K Jolliffe, and I A Wilson
A synthetic peptide that is a bombesin receptor antagonist.
January 1984, Nature,
O Livnah, and D L Johnson, and E A Stura, and F X Farrell, and F P Barbone, and Y You, and K D Liu, and M A Goldsmith, and W He, and C D Krause, and S Pestka, and L K Jolliffe, and I A Wilson
A synthetic peptide derivative that is a cholecystokinin receptor antagonist.
May 1987, The Journal of biological chemistry,
O Livnah, and D L Johnson, and E A Stura, and F X Farrell, and F P Barbone, and Y You, and K D Liu, and M A Goldsmith, and W He, and C D Krause, and S Pestka, and L K Jolliffe, and I A Wilson
Dimerization of the p185neu transmembrane domain is necessary but not sufficient for transformation.
February 1997, Oncogene,
O Livnah, and D L Johnson, and E A Stura, and F X Farrell, and F P Barbone, and Y You, and K D Liu, and M A Goldsmith, and W He, and C D Krause, and S Pestka, and L K Jolliffe, and I A Wilson
Anti-Epo receptor antibodies do not predict Epo receptor expression.
March 2006, Blood,
O Livnah, and D L Johnson, and E A Stura, and F X Farrell, and F P Barbone, and Y You, and K D Liu, and M A Goldsmith, and W He, and C D Krause, and S Pestka, and L K Jolliffe, and I A Wilson
Adrenergic α₁ receptor activation is sufficient, but not necessary for phrenic long-term facilitation.
June 2014, Journal of applied physiology (Bethesda, Md. : 1985),
O Livnah, and D L Johnson, and E A Stura, and F X Farrell, and F P Barbone, and Y You, and K D Liu, and M A Goldsmith, and W He, and C D Krause, and S Pestka, and L K Jolliffe, and I A Wilson
Dimerization of glycoprotein Ibα is not sufficient to induce platelet clearance.
February 2016, Journal of thrombosis and haemostasis : JTH,
O Livnah, and D L Johnson, and E A Stura, and F X Farrell, and F P Barbone, and Y You, and K D Liu, and M A Goldsmith, and W He, and C D Krause, and S Pestka, and L K Jolliffe, and I A Wilson
Evidence for an antagonist specific receptor that does not bind mineralocorticoid agonists.
January 1986, Biochemical and biophysical research communications,
O Livnah, and D L Johnson, and E A Stura, and F X Farrell, and F P Barbone, and Y You, and K D Liu, and M A Goldsmith, and W He, and C D Krause, and S Pestka, and L K Jolliffe, and I A Wilson
An antiestrogen: a phosphotyrosyl peptide that blocks dimerization of the human estrogen receptor.
August 1995, Proceedings of the National Academy of Sciences of the United States of America,
O Livnah, and D L Johnson, and E A Stura, and F X Farrell, and F P Barbone, and Y You, and K D Liu, and M A Goldsmith, and W He, and C D Krause, and S Pestka, and L K Jolliffe, and I A Wilson
Receptor signaling: when dimerization is not enough.
January 1999, Current biology : CB,
Copied contents to your clipboard!