Biomaterial Database

Inter- and intra-phage type differentiation of Salmonella enterica subsp. enterica serovar enteritidis isolates using molecular typing methods. 1998

M Weide-Botjes, and B Kobe, and S Schwarz

Institut für Tierzucht und Tierverhalten, Bundesforschungsanstalt für Landwirtschaft (FAL), Celle, Germany.

Seventy-six Salmonella enterica subsp. enterica serovar Enteritidis (S. Enteritidis) isolates which represented 14 phage types and RDNC isolates were investigated by several independent molecular methods. Eleven different plasmid profiles were detected. Plasmid-encoded virulence genes of the spv gene cluster were shown by hybridization with a spvB/C gene probe to be located on plasmids of either 55 kbp or 95 kbp. Ribotyping as performed with gene probes that recognized either the entire rrn operon or 16S rRNA genes allowed no differentiation between the S. Enteritidis isolates. Three different hybridization patterns could be observed by IS200-typing. Macrorestriction analysis of XbaI-, SpeI- and NotI-digested whole cell DNA proved to be the most discriminatory method as confirmed by the calculation of discriminatory indices. Based on the data obtained from plasmid analysis and macrorestriction analyses with three suitable restriction endonucleases, the 76 S. Enteritidis isolates were assigned to 26 different genomic groups. The observation that several genomic groups included isolates of different phage types has suggested that a combination of suitable molecular methods and phage typing is most useful for tracing S. Enteritidis isolates in epidemiological investigations.

UI	MeSH Term	Description	Entries
D002415	Cats	The domestic cat, Felis catus, of the carnivore family FELIDAE, comprising over 30 different breeds. The domestic cat is descended primarily from the wild cat of Africa and extreme southwestern Asia. Though probably present in towns in Palestine as long ago as 7000 years, actual domestication occurred in Egypt about 4000 years ago. (From Walker's Mammals of the World, 6th ed, p801)	Felis catus,Felis domesticus,Domestic Cats,Felis domestica,Felis sylvestris catus,Cat,Cat, Domestic,Cats, Domestic,Domestic Cat
D002645	Chickens	Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.	Gallus gallus,Gallus domesticus,Gallus gallus domesticus,Chicken
D006736	Horses	Large, hoofed mammals of the family EQUIDAE. Horses are active day and night with most of the day spent seeking and consuming food. Feeding peaks occur in the early morning and late afternoon, and there are several daily periods of rest.	Equus caballus,Equus przewalskii,Horse, Domestic,Domestic Horse,Domestic Horses,Horse,Horses, Domestic
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001434	Bacteriophage Typing	A technique of bacterial typing which differentiates between bacteria or strains of bacteria by their susceptibility to one or more bacteriophages.	Phage Typing,Typing, Bacteriophage,Typing, Phage
D012480	Salmonella Infections	Infections with bacteria of the genus SALMONELLA.	Salmonellosis,Infections, Salmonella,Infection, Salmonella,Salmonella Infection,Salmonelloses
D012481	Salmonella Infections, Animal	Infections in animals with bacteria of the genus SALMONELLA.	Animal Salmonella Infection,Animal Salmonella Infections,Infection, Animal Salmonella,Infections, Animal Salmonella,Salmonella Infection, Animal
D012483	Salmonella Phages	Viruses whose host is Salmonella. A frequently encountered Salmonella phage is BACTERIOPHAGE P22.	Salmonella Bacteriophages,Bacteriophage, Salmonella,Bacteriophages, Salmonella,Salmonella Bacteriophage,Salmonella Phage
D013552	Swine	Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).	Phacochoerus,Pigs,Suidae,Warthogs,Wart Hogs,Hog, Wart,Hogs, Wart,Wart Hog