A World-wide population genetic study on the highly polymorphic DNA locus HUMTHO1, was performed using data collected from 48 studies. Also this study reports new allele frequency data of a Punjabi population (n = 125) from North India. This population is in Hardy-Weinberg equilibrium with 77.6% heterozygosity and power of exclusion at 0.538. Allele frequency graphs were used to demonstrate that world populations show a distinguishable ethnic trend at the HUMTHO1 locus. Heterozygosity (H) and power of exclusion (PE) estimates differ with respect to ethnicity. For example, Caucasian populations have the highest average H and PE and tribal populations the lowest. Using correspondence analysis, it is demonstrated that there is an ethnic trend for this locus. Examination of this trend indicates that some alleles at this locus may be more frequent in specific ethnic populations. This study concludes that the HUMTHO1 locus may be used as one of a number of STR loci for ethnic differentiation studies.