The aim of this investigation was to examine glutathione metabolism (concentration of reduced glutathione, the activity of glutathione reductase, glutathione peroxidase, glutathione-S-transferase, gamma-glutamyl transferase) in the tissues of the brain hemispheres in different periods after complicated trauma (V. Kulagin's model). The obtained results show that the trauma causes compression of the soft tissues and therefore induces noticeable changes in glutathione metabolism in the tissues of the brain hemispheres: diminution of the reduced glutathione content early after trauma and its increase in the late period; high activity of glutathione reductase and gamma-glutamyl n-transferase; activation of glutathione-S-transferase after the trauma when peroxidation was the most active.